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pGL1_39

Plasmid

#199046

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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PAJ698

Plasmid

#178947

Purpose

Expresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)

Depositor

Alfonso Jaramillo

Article

Cunliffe et al ACS Synth Biol. 2022 Oct 21;11(10):3207-

Insert

Chimeric Tail Fiber Fusion P2-gpH and S16 gp37

Use

Unspecified

Expression

Bacterial

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_001497877

Plasmid

#198366

Purpose

Express YP_001497877 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001497877 (B681L )

Expression

Bacterial

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_13 [pIP501 ORI]

Plasmid

#198927

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI

Use

Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-pnGFP-Ultra

Plasmid

#157924

Purpose

Peroxynitrite sensor pnGFP-Ultra in mammalian cells (use with pMAH-POLY-eRF1(E55D))

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra

Expression

Mammalian

Promoter

cmv

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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V621 pGEX-4T-LP - Acceptor

Plasmid

#11715

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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5HT6-GCaMP5G

Plasmid

#48340

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

GCaMP5G

Tags

5HT6

Expression

Mammalian

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
pGL1_4

Plasmid

#199036

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2-GFP

Plasmid

#58881

Purpose

AAV expression of ChR2-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pGL0_4 [pIJ101 ORI]

Plasmid

#198921

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SIRT5H158Y-pET15b

Plasmid

#81032

Purpose

Bacterial expression of Human catalytic mutant SIRT5 protein

Depositor

David Lombard

Article

Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

Insert

Sirt5 (SIRT5 Human)

Tags

His

Expression

Bacterial

Mutation

H158Y catalytic mutant

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187089

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGL0_22 [pSG5 ORI]

Plasmid

#198935

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

Plasmid

#187091

Purpose

GABA sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-iGABASnFR

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR

Plasmid

#187092

Purpose

GABA sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-iGABASnFR

Tags

iRFP670-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187090

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Tags

iRFP670-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGL0_19 [panARS ORI]

Plasmid

#198932

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

panARS ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUCdeltaSS-ERE

Plasmid

#108217

Purpose

Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

Depositor

Didier Picard, Keith Yamamoto

Article

Picard et al Nature 1990 Nov 8;348(6297):166-8.

Insert

ERE-CYC1-lacZ

Expression

Yeast

Promoter

CYC1

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pa-G-GECO1.2

Plasmid

#67622

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-G-GECO1.2

Expression

Mammalian

Mutation

V81H, M84K, L189F, T190S

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.hSynapsin.mCherry-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187102

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Use

AAV

Tags

mCherry-P2A

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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