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2HA-pDEST-N

Plasmid

#118373

Purpose

Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-N

Plasmid

#118368

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Publication

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD3

Plasmid

#21402

Depositor

Eric Metzen

Publication

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN3 (EGLN3 Human)

Tags

EGFP

Expression

Mammalian

Available since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBS MCK CRE

Plasmid

#12529

Depositor

Ronald Kahn

Publication

Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Insert

Cre recombinase

Use

Cre/Lox; Transgenic construct

Mutation

Cre is driven by the Muscle Creatine Kinase promo…

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMpGE013

Plasmid

#108681

Purpose

All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

Depositor

Takayuki Kohchi, Shigeo Sugano

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RCASBP-AP (A) (CC#37)

Plasmid

#15160

Depositor

Connie Cepko

Publication

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Publication

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pQTEV- PRDX2

Plasmid

#31338

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PRDX2 (PRDX2 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Publication

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Publication

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Human, Mouse)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PcDNA3.1-Flag

Plasmid

#210342

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Publication

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

Flag tag

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PMXS-SLC2A3

Plasmid

#72877

Purpose

expresses SLC2A3 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pLVX-M-Flag-BAP1-C91A

Plasmid

#125841

Purpose

express mutant BAP1-C91A

Depositor

Boyi Gan

Publication

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

C91A

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Publication

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1(-)-Cygnet2.1

Plasmid

#19737

Depositor

Wolfgang Dostmann

Publication

Honda et al Proc Natl Acad Sci U S A. 2001 Feb 27. 9

Insert

Cygnet 2.1

Expression

Mammalian

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Publication

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p5E-hPGK

Plasmid

#82579

Purpose

5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Human PGK promoter

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMMB67EH-Spy

Plasmid

#90102

Purpose

IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

Depositor

Samuel Miller

Publication

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YcgR (ycgR )

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA4-Flag-XPA

Plasmid

#22895

Depositor

Aziz Sancar

Publication

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

XPA (XPA Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article