We narrowed to 1,282 results for: membrane gfp

1. pDS240

   Plasmid

   #34966

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Mutation

   K533∆, high-affinity epitope

   Promoter

   GAL1

   Available Since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mCh-kCAAX

   Plasmid

   #112267

   Purpose

   ITRACK Acceptor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   mCherry with KRas membrane targeting domain
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-GCaMP6s-CAAX

   Plasmid

   #52228

   Purpose

   Plasma membrane targeted GCaMP6s, calcium sensor

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   GCaMP6s-CAAX
   

   Tags

   6xHis and CAAX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Lyn-GR-AKAR3

   Plasmid

   #173016

   Purpose

   Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Lyn-GR-AKAR3
   

   Tags

   EGFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lck-mTurquoise2

   Plasmid

   #98822

   Purpose

   In vivo visualization of the plasma membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Lck
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN1-Lck-GCaMP2

   Plasmid

   #24794

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

   Insert

   Lck-GCaMP2
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Myc-CD4-mCherry-HA-FRB

   Plasmid

   #100740

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD4
   

   Tags

   FRB, HA, Myc, and mCherry

   Expression

   Mammalian

   Mutation

   Engineered to remove large part of extracellular …

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PM-APEX2

   Plasmid

   #215557

   Purpose

   Stable, inducible expression in plasma membrane

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   Lyn11-EGFP-Flag tag-APEX2-NES
   

   Use

   Lentiviral

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-PM-ExRai-AktAR2

   Plasmid

   #184050

   Purpose

   Plasma membrane-targeted ExRai-AktAR2

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   PM-ExRai-AktAR2
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lyso-ExRai-AMPKAR

    Plasmid

    #192449

    Purpose

    ExRai-AMPKAR targeted to the lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Lyso-ExRai-AMPKAR
    

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Budding Yeast, Rat)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SNAP-Sec61B

    Plasmid

    #141152

    Purpose

    Fluorescent labeling of ER membrane in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    L79M on Sec61B

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lck-mTurquoise2-iLID

    Plasmid

    #176125

    Purpose

    Plasma membrane localized iLID

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    iLID
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Lyso-ExRai-AktAR2

    Plasmid

    #184052

    Purpose

    Lysosome-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-ExRai-AktAR2
    

    Tags

    Lysosome-associated membrane protein 1 (LAMP1).

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRluc-LC3G120A

    Plasmid

    #105003

    Purpose

    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Glycine 120 mutated to alanine, prevents lipidati…

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-HA-GlpG

    Plasmid

    #155135

    Purpose

    Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid

    #190236

    Purpose

    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid

    #192448

    Purpose

    ExRai-AMPKAR targeted to the mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Mito-ExRai-AMPKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVTCL2

    Plasmid

    #234920

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and plasma membrane-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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