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Plasmid#136512PurposeMammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tagDepositorInsertLyn nano nano 3HA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SH4-FRB-mRFP
Plasmid#100741PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertFyn
UseTagsFRB and mRFPExpressionMammalianMutationSH4 domain onlyPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mitoT
Plasmid#174519PurposeEngineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cellsDepositorInsertMitoT synthetic tether
UseTags6xHis and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDS240
Plasmid#34966PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationK533∆, high-affinity epitopePromoterGAL1Available sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-CCO-IRES-Puromycin
Plasmid#134861PurposeExpresses EGFP-tagged inner mitochondrial membrane markerDepositorInsertcytochrome c oxidase subunit VIII (COX8A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCh-kCAAX
Plasmid#112267PurposeITRACK Acceptor ConstructDepositorInsertmCherry with KRas membrane targeting domain
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-GR-AKAR3
Plasmid#173016PurposePlasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.DepositorInsertLyn-GR-AKAR3
UseTagsEGFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1-Lck-GCaMP2
Plasmid#24794DepositorInsertLck-GCaMP2
UseTagsHis and XpressExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
UseTagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
PM-APEX2
Plasmid#215557PurposeStable, inducible expression in plasma membraneDepositorInsertLyn11-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AMPKAR
Plasmid#192449PurposeExRai-AMPKAR targeted to the lysosomal membrane.DepositorInsertLyso-ExRai-AMPKAR
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-ExRai-AktAR2
Plasmid#184050PurposePlasma membrane-targeted ExRai-AktAR2DepositorInsertPM-ExRai-AktAR2
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-OMP25
Plasmid#141149PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorInsertOMP25 (Synj2bp Budding Yeast, Rat)
UseTagsSNAP tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Sec61B
Plasmid#141152PurposeFluorescent labeling of ER membrane in mammalian cellsDepositorInsertSec61 beta (SEC61B Human)
UseTagsSNAP tagExpressionMammalianMutationL79M on Sec61BPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lck-mTurquoise2-iLID
Plasmid#176125PurposePlasma membrane localized iLIDDepositorInsertiLID
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AktAR2
Plasmid#184052PurposeLysosome-targeted ExRai-AktAR2.DepositorInsertLyso-ExRai-AktAR2
UseTagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HA-GlpG
Plasmid#155135PurposeExpresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1DepositorInsertGlpG (glpG Escherichia coli)
UseTagsGST and HAExpressionBacterialMutationPromotertacAvailable sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRluc-LC3G120A
Plasmid#105003PurposeMammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.DepositorInsertLC3B (Map1lc3b Rat)
UseLuciferaseTagsRluc with C124A substitutionExpressionMammalianMutationGlycine 120 mutated to alanine, prevents lipidati…PromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]
Plasmid#190236PurposeAAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinaseDepositorInsertEGFP
UseAAVTagsF2A, LDLR C-terminal sequence, mRFP1, myristoylat…ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only