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Arrb1-RFP

Plasmid

#35401

Depositor

Robert Lefkowitz

Article

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

RFP

Expression

Mammalian

Promoter

T7

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pMAL-C2X dAmp/Kana+

Plasmid

#154352

Purpose

Empty backbone vector

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Type

Empty backbone

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-RHOA

Plasmid

#133068

Purpose

Expresses RHOA GTPase with tripartite split GFP10

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

ras homolog family member A (RHOA Human)

Tags

GFP10 (split-GFP tripartite)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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HA-mDyn2 pcDNA3

Plasmid

#36264

Depositor

Pietro De Camilli

Article

Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

Insert

Dynamin 2 (Dnm2 Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 20, 2012

Availability

Academic Institutions and Nonprofits only
pET28b-GBP-MTn

Plasmid

#154363

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

His

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL066

Plasmid

#124897

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

3xFlag

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pYTRW26K_1Ti1

Plasmid

#177292

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shIAPEz_2

Plasmid

#185017

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_IAPEz

Use

Lentiviral

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shIAPEz_1

Plasmid

#185016

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_IAPEz

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-S81A

Plasmid

#171241

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser81Ala (AR-S81A)

Promoter

CMV

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Junb

Plasmid

#178441

Purpose

doxycycline-inducible expression of Mouse Junb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Junb (Junb Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMCherry F8-2

Plasmid

#186034

Purpose

Express VHH F8-2 as a mCherry fusion in mammalian cells

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Tags

mCherry

Expression

Mammalian

Promoter

CMV immediate early

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shRFP

Plasmid

#110940

Purpose

Tet-inducible shRNA targeting RFP

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shRFP

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAV106-pTDH3-mCherry-10xSumo-6xSIM

Plasmid

#116928

Purpose

Yeast integrative vector for expression of the SUMO-SIM complex

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10xSUMO-6xSIM

Tags

mCherry

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL031

Plasmid

#137900

Purpose

STU2.0 SpyCas9 tRNA system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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TC1614

Plasmid

#164873

Purpose

CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A inserted into RfxCas13d (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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