We narrowed to 40,141 results for: INO

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
L-YARS2-9

Plasmid

#166533

Purpose

scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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L-YARS2-9

Plasmid

#166533

Purpose

scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA (murine)

Plasmid

#130915

Purpose

Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN murine

Tags

HA

Expression

Mammalian

Mutation

murine cGAS catalytic domain (aa148-507)

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA (murine)

Plasmid

#130915

Purpose

Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN murine

Tags

HA

Expression

Mammalian

Mutation

murine cGAS catalytic domain (aa148-507)

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pChlr-8CQ

Plasmid

#52171

Purpose

Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 284-319 (PUM1 Human)

Expression

Bacterial

Mutation

Changed 12N to 12C in module 8

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pChlr-8CQ

Plasmid

#52171

Purpose

Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 284-319 (PUM1 Human)

Expression

Bacterial

Mutation

Changed 12N to 12C in module 8

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
SNX11-PX (7-167)

Plasmid

#119092

Purpose

Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX11-PX (7-167) (SNX11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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SNX11-PX (7-167)

Plasmid

#119092

Purpose

Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX11-PX (7-167) (SNX11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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O-MARS-11

Plasmid

#166541

Purpose

scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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O-MARS-11

Plasmid

#166541

Purpose

scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only