We narrowed to 25,057 results for: Nes

1. double_sgRNA targeting e3 and e7

   Plasmid

   #190689

   Purpose

   sgRNAs targeting enhancer 3 and 7 of MYC separately

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNAs targeting enhancer 3 and 7 of MYC separately
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCerulean3-Zyxin-6

   Plasmid

   #55454

   Purpose

   Localization: Focal Adhesions, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCRRNA-G0-R0

   Plasmid

   #101803

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPJ173(JPUB_006777)

   Plasmid

   #83834

   Purpose

   Carries NEW operon 10 final

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Inserts

   Kuste3336
   Kuste3339
   Kuste3341
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   op10_3339_for, op10_3341_for, and op10_BsaI_3336_…

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPJ174(JPUB_006779)

   Plasmid

   #83827

   Purpose

   Carries operon 1 final

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Inserts

   Kuste3603
   kuste3605
   kuste3606
   

   Use

   Pfab217 (p15a ori, kanr)

   Tags

   Expression

   Mutation

   Promoter

   op1_3603_for, op1_3605_for, and op1_3606_for

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMV:Flag-dKeNLuc_SV40:PuroR

   Plasmid

   #183046

   Purpose

   Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   dKeNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_CMV:Flag-KaNLuc_SV40:PuroR

   Plasmid

   #183047

   Purpose

   Expresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   KaNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS NL4-3 envFS eGFP Δgag/pol

   Plasmid

   #101343

   Purpose

   Deletion from the start of gag until 229 bases before the cPPT

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta GagPol

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

   Plasmid

   #69903

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG3H-U6EC1

    Plasmid

    #134758

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQTEV-SARA1

    Plasmid

    #34809

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    SARA1 (SAR1A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBacPak-HA-E(y)2

    Plasmid

    #52288

    Purpose

    Expresses Drosophila E(y)2

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    E(y)2 (e(y)2 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGW-FL-V5

    Plasmid

    #78858

    Purpose

    DULIP Prey Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGW-RL-PA

    Plasmid

    #78856

    Purpose

    DULIP Bait Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Renilla luciferase, Protein A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSuper mouse Drosha3

    Plasmid

    #14776

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Drosha shRNA (Drosha Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+) EPHB4 Sense

    Plasmid

    #60638

    Purpose

    Expresses the human EPHB4 exon 11/12 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Ephrin type B receptor 4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTrichoGate-11

    Plasmid

    #160711

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAV1-D67G-HA

    Plasmid

    #27706

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutant D67G

    Promoter

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. MD~E-pCLBabe

    Plasmid

    #20912

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    MyoD~E12 forced dimer
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Short flexible linker region between the MyoD and…

    Promoter

    Available Since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only