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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,083 results for: omp

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Showing: 11781 - 11800 of 21083 results

  1. pET28a_6HT-TBP6.7(R49A)

    Plasmid
    #112726
    Purpose
    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.
    Depositor
    Joseph Wedekind
    Article
    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64
    Insert
    6His-TEV-TBP6.7(R49A)
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    R49A
    Promoter
    T7
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112726-plasmid-map-sequence-id-216329

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  12. PumU

    Plasmid
    #87322
    Purpose
    PumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind U
    Promoter
    UBC
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    87322-plasmid-map-sequence-id-181390

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  13. PumG

    Plasmid
    #87320
    Purpose
    PumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind G
    Promoter
    UBC
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87320-plasmid-map-sequence-id-181386

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  14. PumA

    Plasmid
    #87319
    Purpose
    PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind A
    Promoter
    UBC
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87319-plasmid-map-sequence-id-181378

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  18. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201022-plasmid-map-sequence-id-394460

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Showing: 11781 - 11800 of 21083 results