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pAC-cwo

Plasmid

#33155

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Insert

cwo (cwo Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pBS Alpha 2 globin

Plasmid

#19231

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 globin (HBA1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pMAD-Gate4

Plasmid

#25943

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate5

Plasmid

#25944

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate6

Plasmid

#25945

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMW96_6xHis-Sumo-TEV-mNG

Plasmid

#253320

Purpose

bacterial expression and purification of mNG

Depositor

Kristen Verhey

Article

Geng et al EMBO J. 2024 Aug;43(15):3192-3213. doi:

Insert

mNG

Tags

His-SUMO

Expression

Bacterial

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3_T20-mSGH-AKAR3EV

Plasmid

#236210

Purpose

Fluorescent biosensor for PKA activity proximal to mitochondria

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

Tomm20-QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mBJH-AKAR3EV

Plasmid

#236206

Purpose

Fluorescent biosensor for cytosolic PKA activity

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mSGH-AKAR3EV

Plasmid

#236207

Purpose

Fluorescent biosensor for cytosolic PKA activity

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-S-13xLexAop-GCaMP8m_VK05

Plasmid

#238557

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Sparse (S) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

Currier et al Cell. 2025 Aug 7;188(16):4366-4381.e14.

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05

Plasmid

#238556

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Intermediate (I) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

Currier et al Cell. 2025 Aug 7;188(16):4366-4381.e14.

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015910

Plasmid

#226287

Purpose

Expresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Isoeugenol synthase 1

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR90

Plasmid

#203914

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

GSC2 plus flanking (GSC2 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR89

Plasmid

#203423

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

EGC3 plus flanking (EGC3 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGL6000

Plasmid

#216797

Purpose

Platform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinase

Depositor

Jeremy Mottram

Article

Santos et al Mol Biochem Parasitol. 2017 Sep;216:45-4

Type

Empty backbone

Use

Cre/Lox; Leishmania dicre inducible expression

Tags

6xHA

Promoter

rRNA promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Tags

V5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Tags

V5-APEX2

Expression

Yeast

Promoter

pOM14

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC293-P4B1

Plasmid

#203134

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC293

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC293

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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