We narrowed to 25,057 results for: Nes

1. pFA6a-GFP (F64L, S65T, V163A)-kanMX6

   Plasmid

   #53183

   Purpose

   chromosomal tagging with stable GFP

   Depositor

   John Pringle

   Article

   Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

   Insert

   kanMX6
   

   Use

   Yeast genomic targeting

   Tags

   GFP (F64L, S65T, V163A)

   Expression

   Mutation

   Promoter

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBALU12

   Plasmid

   #69328

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   gSL2-NLS-mChOint-FgF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pO-macs-ISH

   Plasmid

   #15856

   Depositor

   Peter Mombaerts

   Article

   Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

   Insert

   Olfactory specific medium-chain acyl CoA synthetase (Acsm4 Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pIS1 Cyclin D2 short UTR

   Plasmid

   #21644

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 short UTR (CCND2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD3-MS2tail-Scaffold

   Plasmid

   #78906

   Purpose

   Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNLRP11minor.MYC

   Plasmid

   #131195

   Purpose

   Mammalian expression of human NLRP11 188A

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   NLRP11 188A (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   188A

   Promoter

   pCMV

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. UGPC

   Plasmid

   #138954

   Purpose

   Lentiviral vector for co-expressing Cre recombinase and sgRNA

   Depositor

   Nadya Dimitrova

   Article

   Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and PGK

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGC001

   Plasmid

   #48824

   Purpose

   Provides WUS as GreenGate coding sequence module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   WUS (WUS Mustard Weed)
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   internal BsaI recognition site removed by silent …

   Promoter

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7 Mor2 shRNA

   Plasmid

   #59298

   Purpose

   3rd generation lentiviral vector expressing shRNA to mouse Opmr1

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

   Insert

   Mor2 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pMOD_A3601

    Plasmid

    #91051

    Purpose

    Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    dAtCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Myc-CICf_L40S

    Plasmid

    #48187

    Purpose

    Expresses L40S mutated Cic

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Capicua (Cic Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T1 human APC-2,3 (1362–1540)

    Plasmid

    #132631

    Purpose

    expresses human APC-2,3 (1362–1540) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

    Insert

    APC-2,3 (1362–1540)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-PK1Dexp

    Plasmid

    #130834

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKG1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    June 19, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMSCV Cyclin D2-long-mut

    Plasmid

    #21657

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-15/16 and let-7 binding sites

    Promoter

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV Lex VP16 (F442A) HA (P#1710)

    Plasmid

    #14595

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Promoter

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pQTEV-MGC20398

    Plasmid

    #34825

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    MGC20398 (ZNF830 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTH651-RLuc/minFLuc

    Plasmid

    #29697

    Depositor

    Tobias von der Haar

    Article

    Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    Available Since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - C3orf17 sgRNA 1

    Plasmid

    #70652

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C3orf17
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-M2-TLR2-FW/AA

    Plasmid

    #12293

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I, H202Y, F774A and W775A; signal peptide (aa1…

    Promoter

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

    Plasmid

    #50896

    Purpose

    Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L717C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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