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pENTR_BIP-sfGFP-TurboID-KDEL

Plasmid

#240223

Purpose

Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H548A

Plasmid

#238319

Purpose

ScVip1-536-1107-H548A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H548A

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/Raf2/BSD2

Plasmid

#229515

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107

Plasmid

#238312

Purpose

ScVip1-536-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A

Plasmid

#238329

Purpose

ScVip1-536-1107-K554A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A

Plasmid

#238318

Purpose

ScVip1-536-1107-R547A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E991A

Plasmid

#238321

Purpose

ScVip1-536-1107-E991A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E991A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H651A

Plasmid

#238323

Purpose

ScVip1-536-1107-H651A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H651A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAL007

Plasmid

#233866

Purpose

MoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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ER-frNFAST

Plasmid

#233599

Purpose

Expression of frNFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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PM-frNFAST

Plasmid

#233602

Purpose

Expression of frNFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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UBQ10pro-nlsABACUS2-400n-NosT

Plasmid

#203728

Purpose

Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

Depositor

Alexander Jones

Article

Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

Insert

nlsABACUS2-400n

Use

Synthetic Biology

Tags

Myc

Expression

Plant

Promoter

AtUBQ10pro 1500bp

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Airn

Plasmid

#233266

Purpose

CARGO plasmid containing ~90kb dox-inducible Airn transgene

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Airn (Airn Mouse)

Expression

Mammalian

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231355

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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