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Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector

Plasmid

#114430

Purpose

Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6s, tdTomato, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-R62D

Plasmid

#118379

Purpose

Gateway compatible donor vector with actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

R62D

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T240A

Plasmid

#111457

Purpose

Transient expression of Flag-tagged human Sox9 with T240A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T240A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-mCherry

Plasmid

#103816

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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iMb-Notch-Mosaic (IR99.40)

Plasmid

#99749

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(delta ZF)

Plasmid

#90110

Purpose

Mammalian expression of Flag-MCPIP1(delta ZF)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Mutation

K317G, S318G

Promoter

CMV

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-myl7-cytoBirA-2A-mCherry_Ras

Plasmid

#80060

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSico-shRaptor

Plasmid

#81086

Purpose

conditional expression of an shRNA targeting murine Raptor

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Raptor shRNA (Rptor Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

Plasmid

#75115

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

deleted bromodomain

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
Neo1.a-Fc-His

Plasmid

#72089

Purpose

Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.a (Neo1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 dSAP

Plasmid

#68830

Purpose

Expresses c-myc-tagged human RAD18 deleting SAP domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pMM2-hPMCA4a

Plasmid

#47761

Purpose

mammalian expression of human PMCA4a

Depositor

John Penniston, Emanuel Strehler

Article

Enyedi et al J Biol Chem. 1994 Jan 7;269(1):41-3.

Insert

PMCA4a (ATP2B4 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-ERK5-AEF

Plasmid

#31816

Depositor

Astar Winoto

Article

Sohn et al Mol Cell Biol. 2005 Oct;25(19):8553-66.

Insert

Erk5 (Mapk7 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

T219A and Y221F

Promoter

PCMV-LTR

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKR

Plasmid

#23452

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKR (EIF2AK2 Human)

Use

Gateway Cloning

Mutation

aa494-511 missing

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Sup35 ScNM A230C His7

Plasmid

#15598

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Budding Yeast)

Tags

7xHis

Expression

Bacterial

Mutation

A230C. Alanine 230 mutated to cysteine.

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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Anti-GST [N100/13R ] (Recombinant Antibody)

Antibody

#192032-rAb

Purpose

Anti-GST recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Recommended Applications

Western Blot

Reactivity

Synthetic

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Jan. 27, 2023

Availability

Industry, Academic Institutions, and Nonprofits
HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro

Plasmid

#25955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

Plasmid

#208163

Purpose

AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCreR297T

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

Plasmid

#214881

Purpose

Lentiviral vector encoding RfxCas13d targeting B2M guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crB2M-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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