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We narrowed to 63,901 results for: cloning

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Showing: 11961 - 11980 of 63901 results

  1. pLVCT-DEST-IPG

    Plasmid
    #106349
    Purpose
    Destination vector for gateway cloning. Bicistronic expression of Puro-R fused with EGFP under the control of IRES. Tet elements are inserted in 3'LTR for tTR-KRAB mediated gene suppression.
    Depositor
    Thomas Tuschl
    Type
    Empty backbone
    Use
    Gateway Cloning and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    106349-plasmid-map-sequence-id-201450

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  2. pLVE-DEST-IPG

    Plasmid
    #106350
    Purpose
    Destination vector for gateway cloning. Bicistronic expression of Puro-R fused with EGFP followed by murine Eef1a1 3'UTR under the control of IRES. loxP sequence is inserted in 3'LTR.
    Depositor
    Thomas Tuschl
    Type
    Empty backbone
    Use
    Gateway Cloning and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    106350-plasmid-map-sequence-id-201458

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  3. pAG CMV C-HA Puro

    Plasmid
    #237880
    Purpose
    Empty mammalian expression plasmid for cloning C-terminally HA-tagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237880-plasmid-map-sequence-id-469913

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  4. pX458-tRNA-SpCas9

    Plasmid
    #195183
    Purpose
    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.
    Depositor
    Stephanie Roessler
    Insert
    Cas9
    Use
    CRISPR
    Promoter
    tRNA-Gln
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195183-plasmid-map-sequence-id-423143

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  10. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Publication
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  11. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

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  12. p663-UBC-miniTurbo-V5-CDK14_IDG-K

    Plasmid
    #158172
    Purpose
    Gateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK14 (CDK14 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    158172-plasmid-map-sequence-id-328049

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  13. pHAGE-CMV-Flag-GFP_IDG-K

    Plasmid
    #135275
    Purpose
    Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-GFP
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    135275-plasmid-map-sequence-id-267207

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  14. 8061-E29

    Plasmid
    #234266
    Purpose
    Gateway ORF Entry clone of mouse Tgfbr2 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Tgfbr2 (Tgfbr2 Mouse)
    Use
    Gateway Cloning
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234266-plasmid-map-sequence-id-485831

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  15. 8061-E31

    Plasmid
    #234268
    Purpose
    Gateway ORF Entry clone of mouse Smad1 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Smad1 (Smad1 Mouse)
    Use
    Gateway Cloning
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234268-plasmid-map-sequence-id-468855

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  16. 8061-E33

    Plasmid
    #234270
    Purpose
    Gateway ORF Entry clone of mouse Smad3 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Smad3 (Smad3 Mouse)
    Use
    Gateway Cloning
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234270-plasmid-map-sequence-id-468854

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  17. pGL3_SATB2

    Plasmid
    #232361
    Purpose
    Enhancer near SATB2, chr2:199202570-199203402 cloned into pGL3
    Depositor
    Joanna Wysocka
    Insert
    SATB2 (SATB2 Human)
    Use
    Luciferase
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    232361-plasmid-map-sequence-id-466704

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  18. pENTR_AtU6p_BsaI_gRNA

    Plasmid
    #225987
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225987-plasmid-map-sequence-id-448010

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  20. Hs.HRAS Q61K

    Plasmid
    #83187
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61K
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83187-plasmid-map-sequence-id-158150

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Showing: 11961 - 11980 of 63901 results