We narrowed to 16,198 results for: grna

1. pREDCas9

   Plasmid

   #71541

   Purpose

   Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   lambda red genes
   plasmid curing system
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS148∙CsR

   Plasmid

   #197858

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Ap/Cb resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_526

   Plasmid

   #184859

   Purpose

   Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

   Plasmid

   #124864

   Purpose

   Mutagenesis of Grin2b

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2b (Grin2b Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK1334

   Plasmid

   #127965

   Purpose

   sgRNA expression vector compatible with CROP-Seq and imaging screens

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT3GEPIR-WT1shRNA

   Plasmid

   #220560

   Purpose

   To inducibly knockdown WT1 or EWSR1::WT1 expression

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   WT1 shRNA (WT1 Human)
   

   Use

   Lentiviral

   Promoter

   TRE3G (TetOP) promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUPERretro-Sirt7 shRNA2

    Plasmid

    #53145

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSUPERretro-Sirt7 shRNA1

    Plasmid

    #53144

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

12. SGEP

    Plasmid

    #111170

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-CETN2

    Plasmid

    #227291

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. RT3GEPIR

    Plasmid

    #111169

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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