1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. D22_Ld_141410

   Plasmid

   #135838

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   DnaJ domain containing protein, putative
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEMS1410

   Plasmid

   #29207

   Purpose

   Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple171 (RAMP3 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC57-Kan_BR32_00141031

   Plasmid

   #121260

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   BR32_00141031
   

   Use

   Golden gate

   Tags

   Expression

   Mutation

   Magnaporthe oryzae

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC57-Kan_BR32_00141021

   Plasmid

   #121259

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   BR32_00141021
   

   Use

   Golden gate

   Tags

   Expression

   Mutation

   Magnaporthe oryzae

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBS1410 GFP-TDP43 F-S

   Plasmid

   #118799

   Purpose

   To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   All F to S in CTD of full-length TDP43

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

   Plasmid

   #123615

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A/K34A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A/K34A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

   Plasmid

   #159859

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PE-SUMO CRYAB

   Plasmid

   #217670

   Purpose

   For expression of SUMO tagged human alpha B crystallin

   Depositor

   Larry David

   Insert

   CRYAB (CRYAB Human)
   

   Use

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC18-H1 RNAi

   Plasmid

   #87355

   Purpose

   RNAi expression vector based on H1 promoter and pUC18 vector backbone

   Depositor

   Saroj Mathupala

   Article

   Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

   Insert

   H1 RNA Promoter from U87MG glioma cells
   

   Use

   RNAi; Shrna expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 RNA

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT-GEM(2)

    Plasmid

    #62894

    Purpose

    Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT-GEM(1)

    Plasmid

    #62893

    Purpose

    Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

    Plasmid

    #62956

    Purpose

    Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

    Plasmid

    #62954

    Purpose

    Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

    Plasmid

    #62955

    Purpose

    Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

    Plasmid

    #62957

    Purpose

    Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4 in three phases
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-KS-attB2-SA(0)-T2A-Gal4

    Plasmid

    #62899

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT-GEM(0)

    Plasmid

    #62891

    Purpose

    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS-KS-attB2-SA(1)-T2A-Gal4

    Plasmid

    #62900

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-KS-attB2-SA(2)-T2A-Gal4

    Plasmid

    #62901

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

    Plasmid

    #62905

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-dVP16AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge