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pTA7001-VirPWT4-GFP

Plasmid

#237533

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Plant

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-VirPWT4

Plasmid

#237517

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca

Plasmid

#237509

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca_Reg1

Plasmid

#237525

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Yeast

Mutation

Removed binding region 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Cs

Plasmid

#237510

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Cs

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:GST-AvrPWT4

Plasmid

#237522

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-RWT4Ca

Plasmid

#237519

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-RWT4Ca_KDup

Plasmid

#237534

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Dup

Expression

Plant

Mutation

Only contains KDup region

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-RWT4Ca

Plasmid

#237518

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:GST-VirPWT4

Plasmid

#237523

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-RWT4Ca_noKDup

Plasmid

#237535

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_noKDup

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLI_TNF

Plasmid

#171179

Purpose

inducible expression of human TNF

Depositor

Veit Hornung

Article

Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

Insert

human TNF

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDIV296

Plasmid

#204687

Purpose

AMA1 plasmid with nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

nat1

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK094-LM-AMA002.0

Plasmid

#171366

Purpose

Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV297

Plasmid

#204820

Purpose

AMA1 plasmid with amdS selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

amdS

Use

Fungal expression

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans trpC promoter

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GST-auxilin(547-910)

Plasmid

#62572

Purpose

Codes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruption

Depositor

Ernst Ungewickell

Article

Holstein et al J Cell Biol. 1996 Nov;135(4):925-37.

Insert

Auxilin

Tags

GST

Expression

Bacterial

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only
pUC19::ZmUBI-RWT4Ca_K217R/K327R

Plasmid

#237511

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_K217R/K327R

Expression

Plant

Mutation

Mutations at kinase active sites (K217R, K327R)

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_Reg2

Plasmid

#237513

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg2

Expression

Plant

Mutation

Removed binding region 2

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_noKDup

Plasmid

#237515

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_noKDup

Expression

Plant

Mutation

Removed KDup region

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-PKD

Plasmid

#237521

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_pseudokinase

Expression

Bacterial

Mutation

Pseudokinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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