We narrowed to 859 results for: golden gate assembly

1. pOGG039

   Plasmid

   #113996

   Purpose

   SC module. Terminator for T7RNAP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Terminator for T7RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pND005

   Plasmid

   #200951

   Purpose

   Yeast two-hybrid destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Yeast two-hybrid

   Tags

   Expression

   Mutation

   Promoter

   GAL4

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA_Auxiliary 4B

   Plasmid

   #102713

   Purpose

   Provides the End-to-End linker 4B which assists Level 2 cloning in Mobius Assembly

   Depositor

   Naomi Nakayama

   Article

   Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

   Insert

   Linker sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXZ13lacZalphaKanR

   Plasmid

   #160230

   Purpose

   Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.

   Depositor

   Ian Duncan

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOGG037

   Plasmid

   #113995

   Purpose

   SC module. Super-folder GFP green fluorescence reporter

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Super-folder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOGG005

   Plasmid

   #113980

   Purpose

   Level 1 high copy number vector for E. coli expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pND003

   Plasmid

   #200949

   Purpose

   Yeast surface display destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Yeast surface display

   Tags

   AGA2, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOGG041

   Plasmid

   #113997

   Purpose

   Level 0 module. Taurine-inducible promoter for Alphaproteobacteria

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Taurine promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pND004

   Plasmid

   #200950

   Purpose

   MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOGG001

    Plasmid

    #113978

    Purpose

    Level 0 module. nptII promoter for constitutive gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    nptII promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOGG050

    Plasmid

    #113999

    Purpose

    SC module. Thermostable beta-galactosidase reporter

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    celB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOGG031

    Plasmid

    #113994

    Purpose

    Level 0 module. IPTG promoter for inducible expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    IPTG-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOGG030

    Plasmid

    #113993

    Purpose

    Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    IPTG-inducible T7RNAP promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOGG082

    Plasmid

    #114000

    Purpose

    Level 0 module. NifH promoter for nodule-specific expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    NifH promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L0I75_tUBQ10_GG5-GG6

    Plasmid

    #219714

    Purpose

    Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG5-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT terminator with added golden gate linkers

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PGR-Blue

    Plasmid

    #68374

    Purpose

    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.

    Depositor

    Nathan Reyna

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit

    #1000000221

    Purpose

    A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

    Depositor

    Eric Cantor

    Available since

    June 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

18. L0I89-pMUTE_GG0-1

    Plasmid

    #219708

    Purpose

    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pMUTE-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L0I74-_tUBQ10_GG4-GG6

    Plasmid

    #219713

    Purpose

    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG4-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT terminator with added golden gate linkers

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L0I87-SPCHPromoter_GG0-1

    Plasmid

    #219707

    Purpose

    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pSPCH-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge