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Plasmid#121706PurposepMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert6x His epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO801: pMVP (L5-L4) 3x FLAG epitope tag
Plasmid#121702PurposepMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert3x FLAG epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380E
Plasmid#100035PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.DepositorInsertRhoGC D380E
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationD380EPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380N
Plasmid#100034PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.DepositorInsertRhoGC D380N
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationD380NPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC cloning vector
Plasmid#10878Purpose3rd generation transfer plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
FUW mCherry-GFP-LC3
Plasmid#110060PurposeTo visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)DepositorInsertmCherry-GFP-LC3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC control
Plasmid#10879PurposeNegative control shRNA vector containing non-hairpin insert.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG1/κ
Plasmid#61883PurposeExpresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer20
Plasmid#44012PurposeTet-inducible lentiviral vector for ORF expressionDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#69496PurposepJL1 expression vector with sfGFPDepositorInsertSuper folder GFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
1136- Desmoplakin-GFP
Plasmid#32227DepositorInsertDesmoplakin (DSP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
WN10150
Plasmid#80443PurposeExpresses dead (908A) AsCpf1DepositorInsertnuclease dead (908A) AsCpf1
UseTagsNLS-3xHAExpressionMammalianMutationnuclease dead (D908A)PromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSLQ10875
Plasmid#183963PurposeCBA-hyperdCas12a-HA-KRAB-mCherryDepositorInsertHyperdCas12a
UseCRISPRTagsHA, KRAB, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCBAAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb-G76V-GFP-IRES-DsRed
Plasmid#225503PurposeExpression of Ub-G76V-GFP along dsRED from one mRNADepositorInsertUb-G76V-GFP
UseTagsGFPExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterCMV promoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG2/κ
Plasmid#61884PurposeExpresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only