We narrowed to 1,583 results for: MB;

1. pGW02 AAV-TBG-Cre-sgP53-sgRNA

   Plasmid

   #192162

   Purpose

   AAV-TBG-Cre-sgP53-sgRNA

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVPSBA01-SARS-CoV2-nsp1 mutant4

   Plasmid

   #192174

   Purpose

   express SARS2-NSP1-mut4

   Depositor

   Sidi Chen

   Article

   Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

   Insert

   express SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVPSBA01-SARS-CoV2-nsp1-truncated

   Plasmid

   #192172

   Purpose

   express SARS2-NSP1-trunc

   Depositor

   Sidi Chen

   Article

   Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

   Insert

   express SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIFU002

   Plasmid

   #174299

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HA and cMyc

   Expression

   Yeast

   Mutation

   N343Q, N501Y

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIFU001

   Plasmid

   #174298

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HA and cMyc

   Expression

   Yeast

   Mutation

   N343Q, E484K

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGG442

   Plasmid

   #165616

   Purpose

   Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
   

   Use

   CRISPR

   Tags

   3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

   Expression

   Yeast

   Mutation

   Corrected homology relative to wild type SpCas9 (…

   Promoter

   TEF1

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

   Plasmid

   #91819

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

   Plasmid

   #91820

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PD-L1-miSFIT-9-G, 15-C

   Plasmid

   #124688

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmirGlo-CALB2-3'UTR delta ARE&mir30

    Plasmid

    #74430

    Purpose

    Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NC11 pCDNA3.1 VENUS WTX K292N

    Plasmid

    #36966

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX K292N (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    K292N

    Promoter

    CMV

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. NC8 pCDNA3.1 VENUS WTX S286D

    Plasmid

    #36963

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX S286D (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    S286D

    Promoter

    CMV

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. NC7 pCDNA3.1 VENUS WTX S286A

    Plasmid

    #36962

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX S286A (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    S286A

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

14. NC10 pCDNA3.1 VENUS WTX T289A

    Plasmid

    #36965

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX T289A (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T289A

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

15. NC9 pCDNA3.1 VENUS WTX S286E

    Plasmid

    #36964

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX S286E (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    S286E

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00021

    Plasmid

    #34183

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG14548 (E(spl)mbeta-HLH Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pLenti CMV Hygro DHFR.dn-cHSF1

    Plasmid

    #134731

    Purpose

    Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    DHFR.dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti CMV Hygro dn-cHSF1

    Plasmid

    #134732

    Purpose

    Lentiviral expression vector for constitutively active dominant-negative HSF1

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti CMV/TO Zeo dn-cHSF1

    Plasmid

    #134734

    Purpose

    Lentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-201, 380−529

    Promoter

    CMV/TO

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162386

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge