We narrowed to 5,224 results for: golden gate

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pBlue-TAL

Plasmid

#49401

Purpose

The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

Depositor

Michal Zurovec

Article

Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

Type

Empty backbone

Use

TALEN

Expression

Insect

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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CyanoGate Kit

Plasmid Kit

#1000000146

Purpose

Vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

Depositor

Alistair McCormick

Application

Cloning and Synthetic Biology

Vector Type

Bacterial Expression

Cloning Type

Golden Gate (MoClo)

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only
pYPQ148

Plasmid

#69300

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1050

Plasmid

#209540

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Feb. 9, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1051

Plasmid

#209541

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1056

Plasmid

#209546

Purpose

BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 4 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNL2119 pARF7 GG0-GG1

Plasmid

#195910

Purpose

Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF7 (NPH4 Mustard Weed)

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNL2080 pGATA23 GG0-GG1

Plasmid

#195908

Purpose

Level 0 pGATA23 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pGATA23

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I39 PhiC31-Intein GG1-GG4

Plasmid

#195905

Purpose

Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31-intein

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I32 pLBD16 GG0-GG1

Plasmid

#195903

Purpose

Level 0 pLBD16 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pLBD16

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I9 PhiC31 GG1-GG4

Plasmid

#195892

Purpose

Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I40 Intein-PhiC31 GG1-GG4

Plasmid

#195906

Purpose

Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Intein-PhiC31

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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BCJJ223

Plasmid

#117509

Purpose

AtMGE1 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pMGE1:AATG:BsaI

Expression

Plant

Promoter

AtMGE1

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12021

Plasmid

#117511

Purpose

AtICU2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pICU2:AATG:BsaI

Expression

Plant

Promoter

AtICU2

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ433

Plasmid

#117507

Purpose

AtEC1.2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC1.2:AATG:BsaI

Expression

Plant

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ133D

Plasmid

#69289

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLOXp-GUS

Plasmid

#154059

Purpose

Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

beta-glucoronidase (E. coli)

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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