We narrowed to 3,816 results for: golden gate
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Plasmid#120968PurposeMoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAiiA degradase (Bennett, no ssra tag)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P57m
Plasmid#120949PurposeMoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertpCin Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS1604
Plasmid#239528PurposeExpress Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertScYea4p-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1606
Plasmid#239529PurposeExpress Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertScYea4p-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest_PANCEd_AraC_S170_LOV_sd2_WT
Plasmid#242151PurposeExpression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170DepositorInsertAraC S170 LOV
ExpressionBacterialAvailable SinceAug. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1173
Plasmid#239536PurposeExpress CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER markerDepositorInsertmTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN4
Plasmid#233521PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1-10Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1620
Plasmid#239551PurposeExpress GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrCPR-mTagBFP2-2xCrk_SH3
ExpressionYeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1617
Plasmid#239548PurposeExpress GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrG8H-mTagBFP2-2xCrk_SH3
ExpressionYeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1618
Plasmid#239549PurposeExpress GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrG8H-mTagBFP2-2xCrk_SH3
ExpressionYeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1172
Plasmid#239535PurposeExpress CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER markerDepositorInsertCpPT1(52-408)-mTurquoise2
ExpressionYeastAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1619
Plasmid#239550PurposeExpress GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrCPR-mTagBFP2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1601
Plasmid#239526PurposeExpress CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsert2xCrk_SH3-mTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1531
Plasmid#239521PurposeExpress truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPRDepositorInserttVoGES-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1575
Plasmid#239523PurposeExpress Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2DepositorInsertScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1337
Plasmid#239541PurposeExpress Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2DepositorInsertScYea4p-mTurquoise2-ScPex15(315-383)
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1574
Plasmid#239522PurposeExpress Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2DepositorInsertScPex22(1-36)-ScYea4p-mTurquoise2
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3W4Q
Plasmid#234236PurposeHeterologous protein expression of beta-lactamase in Escherichia coliDepositorInsert3W4Q
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3ILS
Plasmid#234215PurposeHeterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coliDepositorInsert3ILS
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5NJO
Plasmid#234202PurposeHeterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coliDepositorInsert5NJO
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits