We narrowed to 5,538 results for: pcas

1. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

   Plasmid

   #107726

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-sREACh-Actin

   Plasmid

   #21949

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

   Insert

   betaActin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pCAGGS-4EBP1 F113A

   Plasmid

   #81122

   Purpose

   expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

   Insert

   4EBP1 F113A (Eif4ebp1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F113A mutation

   Promoter

   CAG

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-Neo-PuroDTK

   Plasmid

   #84036

   Purpose

   PiggyBac cassette contains Neomycin, puromycin and TK genes

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-cofilin S3A

   Plasmid

   #163604

   Purpose

   Expresses constitutively active cofilin in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   cofilin1 (Cfl1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S3A substitution to make cofilin constitutively a…

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG1.1-TSSK2/3xFLAG

   Plasmid

   #199625

   Purpose

   Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tssk2 mRNA (Tssk2 Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAFNF-ECFP-Actb

   Plasmid

   #169787

   Purpose

   An actin FRET sensor. Must be expressed with Venus-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-Venus-MDGA1

   Plasmid

   #82543

   Purpose

   Overexpression vector for Venus-MDGA1 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA1 (MDGA1 Human)
   

   Use

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta actin

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Izumo1-1D4

   Plasmid

   #183544

   Purpose

   Expression vector of mouse izumo sperm-egg fusion 1 (Izumo1) tagged with 1D4 at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

   Insert

   izumo sperm-egg fusion 1 (Izumo1 Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-Ace2_D92N_E199V-mRuby3

    Plasmid

    #129257

    Purpose

    Express Ace2_D92N_E199V-mRuby3 in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-AaCas12b-2AeGFP

    Plasmid

    #121945

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-rTetRDBD-GBP1

    Plasmid

    #50791

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    rTetR DNA binding domain-GBP1 fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-B-161;+80pCalb2

    Plasmid

    #66745

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-C1V1-TT-mRuby2-ST

    Plasmid

    #109124

    Purpose

    Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    C1V1-TT-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTrex-b-NLS-hSpCas9

    Plasmid

    #62543

    Purpose

    Expression of Cas9 in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    NLS-hSpCas9
    

    Use

    CRISPR; Trypanosoma cruzi expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Ribo-HX1

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Cre-iBSD

    Plasmid

    #140284

    Purpose

    Expression of Cre-IRES-BSD

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330_Actb sgRNA / hSpCas9

    Plasmid

    #172832

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9(BB)-2A-GFP-G3BP2(1)

    Plasmid

    #136060

    Purpose

    G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G1333 DddAtox-N–dSpCas9

    Plasmid

    #157833

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-GBP7-p65AD

    Plasmid

    #49441

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP7-p65 transactivation domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge