We narrowed to 40,666 results for: gfp

1. pCMV-GFP-CENPBpA-fixed-length-clone-3

   Plasmid

   #120181

   Purpose

   Randomly picked clone 3 of the pCMV-GFP-CENPBpA-fixed-length library

   Depositor

   David Bartel

   Article

   Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

   Insert

   CENPB poly(A) site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

   Plasmid

   #91803

   Purpose

   Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used

   Depositor

   Jeremy Wilusz

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E

   Plasmid

   #125797

   Purpose

   Histone H2B-superfolder GFP and tdTomato fused by unskippable linker

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   H2BsfGFP-tdTomato
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-CMV GFP-LC3B Q116P G120

   Plasmid

   #129290

   Purpose

   Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP(1-233)-mApple(2-236)-HRas(2-189)

   Plasmid

   #133855

   Purpose

   Mammalian expression of HRas N-terminally tagged with EGFP and mApple

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   HRas (HRAS Human)
   

   Use

   Tags

   EGFP and mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

   Plasmid

   #131354

   Purpose

   Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Caspase-1 (C285A)

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

   Plasmid

   #131133

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-EGFP-cMycBirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA, cMyc (N-terminal of BirA*)

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

   Plasmid

   #121803

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + EF1a::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

   Plasmid

   #121807

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + RIP::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid

    #121748

    Purpose

    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    OLLAS epitope tag-IRES2-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCu-GFP-Atg17(416) (L313D I314K)

    Plasmid

    #121409

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 313 to Aspartic acid; Isoleucine 314 to …

    Promoter

    CUP1 promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-loxP-3xpA-sfGFP-tdTomato T2A Q XE

    Plasmid

    #113852

    Purpose

    Superfolder GFP and tdTomato linked by T2A (Q linker)

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE

    Plasmid

    #113843

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by T2A (V linker)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE

    Plasmid

    #113842

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

    Plasmid

    #112106

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T78R L84R T92R V118R

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-VRER-P2A-GFP-PGK-Puro

    Plasmid

    #110862

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-HF1-P2A-GFP-PGK-Puro

    Plasmid

    #110863

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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