We narrowed to 3,078 results for: lum

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pCM-HA-Gas1

Plasmid

#38313

Depositor

Susan Lindquist

Publication

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

Gas1 (GAS1 Budding Yeast)

Tags

2xHA

Mutation

contains promoter region

Promoter

Endogenous

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pYX-C-mScarlet-Zeo-TSC22D2

Plasmid

#222670

Purpose

endogenously tag TSC22D2 with C-terminal mScarlet

Depositor

Jason Moffat

Publication

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

mScarlet

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYX-N-Puro-mNeon-TSC22D4

Plasmid

#222671

Purpose

endogenously tag TSC22D4 with N-terminal mNeon

Depositor

Jason Moffat

Publication

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D4 (TSC22D4 Human)

Tags

mNeon

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Mitofusin-1 scFv [N111/24]

Plasmid

#211603

Purpose

Mammalian Expression Plasmid of Mitofusin-1 scFv. Derived from hybridoma N111/24.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Mitofusin-1 (Rattus norvegicus) recombinant mouse scFv. (Mfn1 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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GFAP scFv [N206A/8]

Plasmid

#199426

Purpose

Mammalian expression of GFAP scFv. Derived from hybridoma N206A/8.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFAP (Human) (GFAP Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 W201E

Plasmid

#186471

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Publication

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

W201E (no more binding to lipid droplets)

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71345

Purpose

Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

hOTC_Cyan Nano-lantern(Ca2+)

Expression

Mammalian

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

Plasmid

#63884

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

Depositor

Brandon Harvey

Publication

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Promoter

EF1a

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Mito

Plasmid

#65670

Purpose

Expresses Mito-CNL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_3xNLS

Plasmid

#65673

Purpose

Expresses NLS-CNL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-MmUbc7/2783

Plasmid

#11490

Depositor

Allan Weissman

Publication

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS

Plasmid

#216761

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

Depositor

Alex Dunn

Publication

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS

Tags

EGFP and mCherry

Expression

Mammalian

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2 Nsp3TM

Plasmid

#204206

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Publication

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

none, from obtained clone

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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WRN scFv [N428/12]

Plasmid

#206784

Purpose

Mammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

WRN (Homo sapiens) recombinant scFV (WRN Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/38]

Plasmid

#206777

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/38 scFv.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112B/14]

Plasmid

#199423

Purpose

Mammalian expression of Kir2.1 K+ channel scFv. Derived from hybridoma N112B/14.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingKir2.1 K+ channel (Rat) (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Publication

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Tags

HA, lifeact, and p2a

Expression

Plant

Promoter

35S

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgULK1-1

Plasmid

#109004

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ULK1-sgRNA #1 (ULK1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111926

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Publication

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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