We narrowed to 4,550 results for: 187
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Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLI_BFP_C-tag TNF
Plasmid#171187Purposeinducible expression of C-tag TNFDepositorInsertC-tag TNF
UseLentiviralTagsC-tag (internal)ExpressionMammalianMutationADAM cleavage site of TNF (AA73-76) was exchanged…Promotertight TRE promoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZH512
Plasmid#102666PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn1b
Plasmid#61874Purposebacterial expression of a GST-Sesn1b (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZH511
Plasmid#102665PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag-Sesn2
Plasmid#61871PurposeLentivirial expression of Flag-Sesn2 (mouse)DepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (D14A/G38A)
Plasmid#128118PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)DepositorInsertCascAID (D14A/G38A)
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ICK_IDG-K
Plasmid#158187PurposeGateway destination clone of ICK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-ICK (CILK1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myc-CICf_L40S
Plasmid#48187PurposeExpresses L40S mutated CicDepositorAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT35S (GB0036)
Plasmid#68187PurposeProvides the CaMV 35S terminator as a level 0 GoldenBraid partDepositorInsertCaMV 35S terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
SP_H1
Plasmid#228187PurposePlasmid contains the secretion signal peptide sequence of the yeast SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P32603_SPR1_YEAST:0-20
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 L113A+
Plasmid#231873PurposeBacterial expression of N-terminally 6His tagged Gcn4 L113A+DepositorInsertGcn4 L113A+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationL113A, E114Y, N126H, D127E, S136APromoterT7Available SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 F108A+
Plasmid#231875PurposeBacterial expression of N-terminally 6His tagged Gcn4 F108A+DepositorInsertGcn4 F108A+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationF108A, T121L, A141PPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoED W
Plasmid#231871PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoED WDepositorInsertGcn4 ILVtoED W
Tags6xHis, TEV cleavage siteExpressionBacterialMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-Cut190*SS
Plasmid#218706PurposeEncodes codon optimized Cut190*SSenzyme for bacterial expressionDepositorInsertCut190*SS
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-SfCut
Plasmid#218708PurposeEncodes codon optimized SfCut enzyme for bacterial expressionDepositorInsertSfCut
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-2H12Matry
Plasmid#221187PurposeHis6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)DepositorInsertcdGreen-Matry
ExpressionBacterialPromoterT7Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only