We narrowed to 12,607 results for: NSI

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Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_TRE-jRCaMP1b

Plasmid

#229798

Purpose

Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

jRCaMP1b

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDSK519-cspB

Plasmid

#207162

Purpose

Expresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vector

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Tags

HA

Expression

Bacterial

Promoter

nptII

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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TtA_pET29b

Plasmid

#215432

Purpose

Expression construct for TtA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

TtA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF29

Plasmid

#200028

Purpose

Lentiviral vector for expression of KSHV ORF29

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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CAG.INBox-mCherry-INBox-eDHFR

Plasmid

#176203

Purpose

Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

INBox-mCherry-INBox-eDHFR

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox-eDHFR

Plasmid

#176202

Purpose

Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBox-eDHFR

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-ORF68

Plasmid

#162659

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-UL52

Plasmid

#162663

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 C415A

Plasmid

#162633

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C415 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 H452A

Plasmid

#162634

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H452 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 C79A

Plasmid

#162635

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C79A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C79 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K435A

Plasmid

#162636

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K435A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 R443A

Plasmid

#162637

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R443A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K450A/R451A

Plasmid

#162638

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 R14A

Plasmid

#162640

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R14A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid R14 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K310A

Plasmid

#162641

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K310A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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