We narrowed to 10,142 results for: SEC

1. MTK234_029

   Plasmid

   #123909

   Purpose

   Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA3 -hThal5.10-2_hg18
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK6_013

   Plasmid

   #123867

   Purpose

   Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   WPRE-Blast (no terminator)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3_018

   Plasmid

   #123733

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   spdCas9(1-204)-frb-D10A-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET20b-YbAnbu

   Plasmid

   #129637

   Purpose

   Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

   Depositor

   Matthias Bochtler

   Article

   Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

   Insert

   YbAnbu
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   V14A and G107H

   Promoter

   T7

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET20b-YbAnbuT1A

   Plasmid

   #129638

   Purpose

   Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

   Depositor

   Matthias Bochtler

   Article

   Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

   Insert

   YbAnbuT1A
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   T1A, V14A and G107H

   Promoter

   T7

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC17

   Plasmid

   #118875

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and tagRFP

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC16

   Plasmid

   #118870

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
   

   Use

   Tags

   EGFP and HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC218

   Plasmid

   #118907

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS10, cleavable with 3C protease, YBBR tag

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOCC18

   Plasmid

   #118868

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease, tagRFP

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC171

    Plasmid

    #118888

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC202

    Plasmid

    #118880

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mKate2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOCC139

    Plasmid

    #118872

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and mGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT mir-1-1 reporter hsa-mir-205

    Plasmid

    #46744

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-205 (MIR205 Human)
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (MT)

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT mir-1-1 reporter hsa-mir-128-1

    Plasmid

    #46743

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-128-1 (MIR128-1 Human)
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (MT)

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHCMM2

    Plasmid

    #167453

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCMM3

    Plasmid

    #167454

    Purpose

    A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-SYN-H-sDarken

    Plasmid

    #184797

    Purpose

    High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    H-sDarken
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-SYN-sDarken

    Plasmid

    #184796

    Purpose

    Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    sDarken
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge