Skip to main content
Addgene
Showing: 1241 - 1260 of 1648 results
  1. pRH3139

    Plasmid
    #210167
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1
    Depositor
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33G
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pRH3142

    Plasmid
    #210168
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1
    Depositor
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    D147G
    Promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Use
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pDule2-para-aminoPhe

    Plasmid
    #85503
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pBA-mKeima-PIM

    Plasmid
    #174650
    Purpose
    Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
    Depositor
    Insert
    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    Chicken beta-actin
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pQCXIP-SHMY-PAUF

    Plasmid
    #182645
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence
    Depositor
    Insert
    Pancreatic adenocarcinoma upregulated factor (PAUF)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1260 of 1648 results