We narrowed to 5,570 results for: donor

1. qTAG-N-Solo-mStayGold-PCNT

   Plasmid

   #227285

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Solo-sTagRFP-TUBA1B

   Plasmid

   #207765

   Purpose

   Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TERTp124_Gluc_CCR5

   Plasmid

   #192271

   Purpose

   Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Mutation

   -124 C to T

   Promoter

   TERT promoter driving expression of gaussia

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TERTp146_Gluc_CCR5

   Plasmid

   #192272

   Purpose

   Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Mutation

   -146 C to T

   Promoter

   TERT promoter driving expression of gaussia

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE_NFL linker(A6TAG)-3xFLAG KI

   Plasmid

   #182680

   Purpose

   Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and chicken beta-actin promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET-62-SFFV-T2A-PuroR-polyA-R11b

   Plasmid

   #179893

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET-64-SFFV-T2A-BlaR-polyA-R11b

   Plasmid

   #179895

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FluoSTEP-AKAR (C/Y)

   Plasmid

   #181862

   Purpose

   FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (C-Y)
   

   Tags

   CFP(1-10) and cpVenus(E127)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 PAK5 P50L

   Plasmid

   #60533

   Purpose

   Donor vector for human Pak5 P50L mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Mutation

   P50L

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223 PAK5 P76T

    Plasmid

    #60534

    Purpose

    Donor vector for human Pak5 P76T mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Mutation

    P76T

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDONR223 PAK5 S312C

    Plasmid

    #60535

    Purpose

    Donor vector for human Pak5 S312C mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Mutation

    S312C

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDONR223 PAK5 V463L

    Plasmid

    #60536

    Purpose

    Donor vector for human Pak5 V463L mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Mutation

    V463L

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDONR223 PAK5 V593I

    Plasmid

    #60539

    Purpose

    Donor vector for human Pak5 V593I mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Mutation

    V593I

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid

    #183078

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    V600E (T>A), S607S (TCC>AGT)

    Promoter

    None for primary insert. PGK for puromycin resist…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GK Rab3A T36N p201

    Plasmid

    #44697

    Depositor

    Geri Kreitzer

    Insert

    Rab3A (RAB3A Human)
    

    Use

    Gateway donor vector

    Mutation

    T37N

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

16. GK Rab27A p201

    Plasmid

    #44705

    Depositor

    Geri Kreitzer

    Insert

    RAB27A (RAB27A Human)
    

    Use

    Gateway donor vector

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

17. GK Rab3A Q81L p201

    Plasmid

    #44698

    Depositor

    Geri Kreitzer

    Insert

    Rab3A (RAB3A Human)
    

    Use

    Gateway donor vector

    Mutation

    Q81L

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDONR SC4MOL 519

    Plasmid

    #27981

    Depositor

    Igor Astsaturov

    Insert

    SC4MOL (MSMO1 Human)
    

    Use

    Gateway donor vector

    Mutation

    H173Q mutation in SC4MOL

    Available Since

    March 2, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Mammalian

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pScarlessHD-DsRed

    Plasmid

    #64703

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    piggyBac-3xP3-DsRed
    

    Promoter

    3xP3

    Available Since

    Dec. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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