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pMaCTag-04

Plasmid

#119983

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pML1

Plasmid

#108332

Purpose

MIDAS Level-1 source vector for cloning of modules (synthetic biology parts)

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMMRSF1-16

Plasmid

#20177

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

src homology domain 3 (CRK Human)

Tags

His and NpuDnaEC16

Expression

Bacterial

Available Since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pTV-Fbx15

Plasmid

#13464

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

IRES-Bgeo-pA cassette flanked by Fbx15 homologous arms

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
XE3 Xwnt-5A Full Length

Plasmid

#16875

Depositor

Randall Moon

Article

Moon et al Development. 1993 Sep. 119(1):97-111.

Insert

Xwnt-5A (wnt5a.L Frog)

Use

Xenopus expression

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
ZE29 Stbm-deltaN-6myc-pCS2+

Plasmid

#17071

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm deltaN (vangl2 Zebrafish)

Use

Zebrafish expression

Tags

Myc6

Mutation

amino acids 240-526 only

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
C413-E31: attB4-TRE3Gp>-attB1r

Plasmid

#162924

Purpose

Gateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTAL1

Plasmid

#31031

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALE

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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