We narrowed to 1,287 results for: Gateway destination

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pBullet-tgn-n

Plasmid

#53071

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Publication

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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PB-Neo-TetO-Cdk13_Nterm_FlagHa

Plasmid

#127345

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Publication

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Piggybac transposase vector

Tags

Flag- HA- Tandem Epitopes

Expression

Mammalian

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

TetO Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBullet-er-n

Plasmid

#53067

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Publication

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Publication

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

Plasmid

#210487

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pKB12 (pDEST-DHFR F[3] C-term, HygR)

Plasmid

#210486

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj10a-IRES-EGFP

Plasmid

#164947

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj10a (kcnj10a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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WPXL LEFTY2 gRNA

Plasmid

#101924

Purpose

LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Publication

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

LEFTY2 enhancer targeting gRNA

Use

Lentiviral

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2-pax3a-kcnj13-IRES-EGFP

Plasmid

#164950

Purpose

Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

pax3a promoter -5.4k

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-IRES-EGFP

Plasmid

#164941

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnk9-IRES-EGFP

Plasmid

#164949

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Publication

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Promoter

ADH1

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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