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Plasmid#222830PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pHEE-R-kan5A9si
Plasmid#222836PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg
Plasmid#222826PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar
Plasmid#222828PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg5A
Plasmid#222829PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar5A
Plasmid#222831PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan9si
Plasmid#222833PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar9si
Plasmid#222834PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg9si
Plasmid#222832PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg5A9si
Plasmid#222835PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar5A9si
Plasmid#222837PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-ECOligA
Plasmid#165564PurposeConnects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.DepositorInsertE. coli DNA Ligase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFGL821
Plasmid#58223PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL821-8
Plasmid#58224PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL822
Plasmid#58225PurposeFor fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.DepositorInsertphosphinothricin acetyl transferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL822-8
Plasmid#58226PurposeFor fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.DepositorInsertphosphinothricin acetyl transferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRm43GW
Plasmid#133748PurposeThree-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA
Plasmid#48884PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFG7m34GW
Plasmid#133747PurposeThree-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGL820
Plasmid#58221PurposeFor fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.DepositorInsertILV2
UseTagsExpressionBacterialMutationPromoterILV2 native promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL820-2
Plasmid#58222PurposeFor fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.DepositorInsertILV2
UseTagsExpressionBacterialMutationPromoterILV2 native promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-7)-neg_tag1
Plasmid#219419PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-7)-neg_tag1
UseTagsDEEEDD and MGHHHHHGGExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG546
Plasmid#214212PurposeArabinose-inducible folA (used in cross-feeding experiment)DepositorInsertAraC, folA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG550
Plasmid#214213PurposeArabinose-inducible trpC (used in cross-feeding experiment)DepositorInsertAraC, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBW004
Plasmid#131805PurposeVector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'DepositorArticleInsertZea mays FNR and Z. mays SIR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNJB80
Plasmid#51518PurposeGateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
oma-1 ORF in L4440
Plasmid#82547Purposeoma-1 RNAi feeding constructDepositorInsertoma-1 ORF
UseTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
oma-2 ORF in L4440
Plasmid#82548Purposeoma-2 RNAi feeding constructDepositorInsertoma-2 ORF
UseTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 MethylAblet
Plasmid#153412PurposeIt is used to bypass the Level 1 cloning and directly feed terminators into Level 2DepositorInsertamilCP
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Antibody#193445-rAbPurposeAnti-GFAP (Human) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsImmunohistochemistry and Western BlotReactivityHuman, Mouse, and RatSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceJan. 30, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm)
Plasmid#48886PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFGL815
Plasmid#52322PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGate-MCY2
Plasmid#47679PurposeBinary vector for Arabidopsis transformation with SapI Golden Gate sites and mCherry seed selectionDepositorTypeEmpty backboneUseArabidopsis transformationTagsExpressionMutationPromoterAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGate-SE7
Plasmid#47676PurposeBinary vector for Arabidopsis transformation with BsaI Golden Gate sites and mCherry seed selection.DepositorTypeEmpty backboneUseArabidopsis transformationTagsExpressionMutationPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T E7 Q
Plasmid#13648DepositorInsertHPV 16 E7 Q mutant (E7 Human)
UseTagsGSTExpressionBacterialMutationaa 30-37 changed from DSSEEEDE to QSSQQQQQ.PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECT3
Plasmid#75450PurposePlasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt3
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm) -R
Plasmid#48888PurposeNegative feedback plasmid from oscillator without luxRDepositorInsertaiiA
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only