We narrowed to 2,770 results for: GUS

1. pHRep-HKH1

   Plasmid

   #232909

   Purpose

   Plasmid for disruption of the phaC1 locus of Cupriavidus necator via homologous recombination

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   KanR (kanR Synthetic)
   

   Use

   Synthetic Biology

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-Rheb

   Plasmid

   #133767

   Purpose

   Expresses GFP-tagged Rheb in mammalian cells

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   Rheb (RHEB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Clomeleon

   Plasmid

   #203723

   Purpose

   A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

   Depositor

   George Augustine

   Article

   Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

   Insert

   cyan fluorescent protein linked yellow florescent protein
   

   Expression

   Bacterial and Mammalian

   Mutation

   Cyan Fluorescent Protein, Topaz Fluorescent Prote…

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKESa

   Plasmid

   #211828

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. punc-119c

   Plasmid

   #21595

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119 (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Expression

   Worm

   Mutation

   N/A

   Available Since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS-21a-PcAad1

   Plasmid

   #163039

   Purpose

   expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

   Depositor

   Jean-Marie Francois

   Article

   Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

   Insert

   aryl-alcohol dehydrogenase
   

   Tags

   GST and His6

   Expression

   Bacterial

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPpT4- PGK1prom-TIR1-FLAG

   Plasmid

   #189724

   Purpose

   Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Expression

   Yeast

   Promoter

   PGK1 promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-RhebL1

   Plasmid

   #133766

   Purpose

   Expresses GFP-tagged RhebL1 in mammalian cells

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   RhebL1 (RHEBL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. punc-119cR

    Plasmid

    #21596

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119:mCherry (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    mCherry

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dapp1-PH pET11a

    Plasmid

    #71376

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Dapp1-PH (DAPP1 Human)
    

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 148-273

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Grp1-PH pET11a

    Plasmid

    #71377

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

    Plasmid

    #91932

    Purpose

    Bacterial expression of maize SUMO1a and SCE1b

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1b-Myc
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

    Plasmid

    #91934

    Purpose

    Bacterial expression of maize SUMO1a and SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d-Myc
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPpT4- TEF2prom-TIR1F74G-FLAG

    Plasmid

    #189727

    Purpose

    Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    TEF2

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Atg11 [699-800] pHis2

    Plasmid

    #190264

    Purpose

    Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

    Insert

    ATG11
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    HTA1

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    TEF2

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

    Plasmid

    #91945

    Purpose

    Bacterial expression of maize SAE1 and truncated SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Tags

    FLAG and HA

    Expression

    Bacterial

    Mutation

    Truncation of ZmSAE2 to mimic a splice variant

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCnRed

    Plasmid

    #232908

    Purpose

    Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    gam (gam phage Lambda, Synthetic)
    

    Use

    Synthetic Biology

    Promoter

    Pbad-rha

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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