We narrowed to 14,270 results for: lenti crispr

1. pLenti6.3-SauriCas9-KKH-BlastR

   Plasmid

   #220972

   Purpose

   Expresses human codon-optimized SauriCas9-KKH

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SauriCas9-KKH
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiGuideB3GNT5-neo

   Plasmid

   #220867

   Purpose

   encodes CRISPR sgRNA targeting human B3GNT5

   Depositor

   Jennifer Kohler

   Insert

   B3GNT5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192665

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti GFAABC1D-dCas9-KRAB-MeCP2

   Plasmid

   #194701

   Purpose

   Expresses dCas9-KRAB-MeCP2 in Astrocytes

   Depositor

   Timothy Jarome

   Article

   Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

   Insert

   GFAABC1D promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   None

   Promoter

   GFAABC1D

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

   Plasmid

   #138460

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-KRAB-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

   Plasmid

   #138461

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-VP64-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

   Plasmid

   #170378

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

   Plasmid

   #157985

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiRCas9-CUG

    Plasmid

    #104183

    Purpose

    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-CUGsgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiRCas9-Lambda2

    Plasmid

    #104184

    Purpose

    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-sgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB700 lenti gRNA KanR neo_TtoC and hairpin extension

    Plasmid

    #167925

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiGuide-BC-start

    Plasmid

    #127168

    Purpose

    Entry plasmid for two-step guide-barcode cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

    Plasmid

    #189633

    Purpose

    Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

    Plasmid

    #189632

    Purpose

    Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge