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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pOD2087-trnDEG

Plasmid

#89369

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).

Depositor

Karen Oegema

Publication

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Pmec-18

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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AIRE_pCSdest

Plasmid

#53891

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

AIRE

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pBS-TarHom-Tol2-kanaR-cryaa-CFP

Plasmid

#74151

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Publication

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-CFP

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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Human 3' HP1a AID GFP PuroR

Plasmid

#127906

Purpose

HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

Depositor

Mark Groudine

Publication

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

HP1 (CBX5 Human, Mustard Weed)

Use

Donor plasmid

Tags

GFP, AID, PuroR

Mutation

Inserting GFP AID 2A Puro into mouse 3' Hp1a

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TccCas13a Twin SUMO

Plasmid

#199754

Purpose

Expresses codon optimized thermostabile TccCas13a in E.coli

Depositor

Magdy Mahfouz

Publication

Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

TccCas13a

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Publication

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Promoter

Plac

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Publication

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Expression

Bacterial

Mutation

NO

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaCC2

Plasmid

#124957

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Publication

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMpGWB335

Plasmid

#68663

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ351 - MCS(cxTi10882, IV)

Plasmid

#34867

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10882 targeting

Use

Worm targeting

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

Plasmid

#44081

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPI138

Plasmid

#113229

Purpose

extrachromosomal expression vector, expression of LifeAct-GFP

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Promoter

act15

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-ΔD-box-CycA-GFP (525)

Plasmid

#39856

Depositor

Jonathon Pines

Publication

den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

Insert

Cyclin A2 (CCNA2 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

L2V; Δ destruction box = deletion of aa47-55

Available since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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NC3 pCDNA3.1 FLAG WTX (1-804)

Plasmid

#36958

Depositor

Randall Moon

Publication

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pLV-CMV-dnMCAK

Plasmid

#205993

Purpose

Over-expression of GFP-dnMCAK

Depositor

Floris Foijer

Publication

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

dnMCAK

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a(+)/FaGH16A

Plasmid

#100910

Purpose

Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

Depositor

Jan-Hendrik Hehemann

Publication

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH16A

Tags

His-Tag

Expression

Bacterial

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-165

Plasmid

#118577

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-mCherry-Ki67

Plasmid

#183750

Purpose

Expresses EGFP-mCherry-Ki67

Depositor

Daniel Gerlich

Publication

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pB_35S/mEGFP

Plasmid

#135320

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

Depositor

Mathias Pribil

Publication

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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