Addgene

We narrowed to 12,857 results for: sequence

Showing: 1301 - 1320 of 12857 results

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pwtCas9-bacteria

Plasmid

#44250

Purpose

aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

wild-type Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE27

Plasmid

#90369

Purpose

Type II Interferon - STAT1/STAT1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

STAT1

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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PGK 4xMito-mEmerald

Plasmid

#200430

Purpose

Mammalian expression of fluorescently-tagged marker for mitochondria

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence

Use

Tags

mEmerald

Expression

Mammalian

Mutation

4x mito-targeting sequence

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pG108-K - AhpC-Glow

Plasmid

#185135

Purpose

Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Inserts

Glow gene
Bacteroides thetaiotaomicron ahpC promoter

Use

Pg106

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CROPseq-Puro-24xMS2

Plasmid

#212629

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Use

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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ePE2

Plasmid

#202029

Purpose

Prime Editing plasmid for tag insertion

Depositor

Pengcheng Wei

Article

Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

Insert

Cas9，

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
AAV-CAG-flex-MCS--4x6T

Plasmid

#196420

Purpose

Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV and Cre/Lox; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE12

Plasmid

#90347

Purpose

FXR - Farnesoid X receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

FXR

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Stat3 Flag pRc/CMV

Plasmid

#8707

Depositor

Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

*see comments

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE13

Plasmid

#90348

Purpose

LXR - Liver X receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

LXR

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE15

Plasmid

#90354

Purpose

RARA - Retinoic acid receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RARE

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-LMNB1-IRES2-mCherry

Plasmid

#159599

Purpose

Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-LMNB1-IRES2-mCherry

Use

Tags

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE14

Plasmid

#90349

Purpose

VDR - Vitamin D receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

VDR

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Article

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Insert

ERK2 (MAPK1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG72QDProGFP p306

Plasmid

#15833

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only