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Showing: 1981 - 2010 of 7088 results

  1. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

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  2. pEF-GalNAcT2-moxGFP2

    Plasmid
    #218966
    Purpose
    Expresses full length GalNAcT2-moxGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-moxGFP2 (GALNT2 Human)
    Use
    Tags
    moxGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218966-plasmid-map-sequence-id-445673

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  3. pAAV hSyn-Flex RG-cerulean

    Plasmid
    #98221
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV
    Tags
    cerulean
    Expression
    Mutation
    Promoter
    human Synapsin
    Available since
    Aug. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98221-plasmid-map-sequence-id-192715

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  4. pLV Agpat2-V5

    Plasmid
    #175159
    Purpose
    Lentiviral expression of mouse Agpat2-V5
    Depositor
    Larry Gerace
    Insert
    Agpat2 (Agpat2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175159-plasmid-map-sequence-id-352271

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  5. pLV Cnep1r1-V5

    Plasmid
    #175133
    Purpose
    Lentiviral expression of mouse Cnep1r1-V5
    Depositor
    Larry Gerace
    Insert
    Cnep1r1 (Cnep1r1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175133-plasmid-map-sequence-id-352020

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  6. pLV H2afy-V5

    Plasmid
    #175122
    Purpose
    Lentiviral expression of mouse Macroh2a1-V5
    Depositor
    Larry Gerace
    Insert
    Macroh2a1 (Macroh2a1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175122-plasmid-map-sequence-id-351930

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  7. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  8. pEF-GalNAcT2-mGold

    Plasmid
    #218964
    Purpose
    Expresses full length GalNAcT2-mGold in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-mGold (GALNT2 Human)
    Use
    Tags
    mGold
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218964-plasmid-map-sequence-id-445690

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  9. pEF-GalNAcT2-msYFP2

    Plasmid
    #218963
    Purpose
    Expresses full length GalNAcT2-msYFP2 in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msYFP2 (GALNT2 Human)
    Use
    Tags
    msYFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218963-plasmid-map-sequence-id-446179

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  10. pEF-GalNAcT2-msGFP2

    Plasmid
    #218965
    Purpose
    Expresses full length GalNAcT2-msGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msGFP2 (GALNT2 Human)
    Use
    Tags
    msGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218965-plasmid-map-sequence-id-446216

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  11. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  12. pLV V5-Eva1b

    Plasmid
    #175158
    Purpose
    Lentiviral expression of V5-tagged mouse Eva1b
    Depositor
    Larry Gerace
    Insert
    Eva1b (Eva1b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175158-plasmid-map-sequence-id-352269

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  13. pLV Cenpv-V5

    Plasmid
    #175125
    Purpose
    Lentiviral expression of mouse Cenpv-V5
    Depositor
    Larry Gerace
    Insert
    Cenpv (Cenpv Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175125-plasmid-map-sequence-id-351968

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  14. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125196-plasmid-map-sequence-id-243601

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  18. pKAM-E-Flag-GFP

    Plasmid
    #101866
    Purpose
    Lentiviral Evi-1-Flag with GFP marker
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    101866-plasmid-map-sequence-id-211119

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  19. pLV Cbx1-V5

    Plasmid
    #175106
    Purpose
    Lentiviral expression of mouse Cbx1-V5
    Depositor
    Larry Gerace
    Insert
    Cbx1 (Cbx1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175106-plasmid-map-sequence-id-351794

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  21. Aiolos AA130-189 in Artichoke

    Plasmid
    #74451
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74451-plasmid-map-sequence-id-140674

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  22. pLV Lnpk-V5

    Plasmid
    #175121
    Purpose
    Lentiviral expression of mouse Lnpk-V5
    Depositor
    Larry Gerace
    Insert
    Lnpk (Lnpk Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    175121-plasmid-map-sequence-id-351819

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  23. ELL1 CtoA

    Plasmid
    #127279
    Purpose
    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHNK by Q5 mutagenesis
    Promoter
    EF1a
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127279-plasmid-map-sequence-id-249989

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  24. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  25. pKAM-E-Flag-BFP

    Plasmid
    #101867
    Purpose
    Lentiviral Evi-1-Flag with dual BFP and puro markers
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101867-plasmid-map-sequence-id-211116

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  26. pKAM-ME-Flag-BFP

    Plasmid
    #101868
    Purpose
    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101868-plasmid-map-sequence-id-211131

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  27. PB-TO-MYOD1-shOct4

    Plasmid
    #182309
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    shOct4
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182309-plasmid-map-sequence-id-359523

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  30. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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Showing: 1981 - 2010 of 7088 results