-
Plasmid#48888PurposeNegative feedback plasmid from oscillator without luxRDepositorInsertaiiA
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AS+
Plasmid#78242PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 BS-
Plasmid#78245PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 BS+
Plasmid#78244PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AS-
Plasmid#78243PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1
Plasmid#75446PurposePlasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGL889
Plasmid#52321PurposeIntroducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXA6 (murine, partial) HIS-tag pET
Plasmid#8558DepositorInsertHOXA6 (Hoxa6 Mouse)
UseTagsHis and T7ExpressionBacterialMutationpartial cDNA encoding homeodomain and preceeding …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pECT1B
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AN-
Plasmid#78247PurposeOne of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AN+
Plasmid#78246PurposeOne of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
phRL-TKlk-lin-41_LCS-sm
Plasmid#26487DepositorInsertlin-41_LCS-sm
UseTagsExpressionMammalianMutationSeed regions of both target sites are mutated.PromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
phRL-TKlk-lin-41_LCS-2U
Plasmid#26543DepositorInsertlin-41_LCS-2U
UseTagsExpressionMammalianMutationSeed regions of both target sites are mutated.PromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
phRL-TKlk-lin-41_LCS-2G
Plasmid#26544DepositorInsertlin-41_LCS-2G
UseTagsExpressionMammalianMutationSeed regions of both target sites are mutated.PromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJS169
Plasmid#48882PurposeNegative feedback plasmid from oscillatorDepositorInsertlacI with ssrA tag
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterPara/lacAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
GGE114
Plasmid#120731PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolyticaDepositorTypeEmpty backboneUseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pxpr_050-wt-ltr
Plasmid#182646Purposelentiviral expression of gRNA scaffold, contains intact LTRDepositorInsertNone
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECM1
Plasmid#75445PurposePlasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-mCherry
Plasmid#23154DepositorInsertmouse gammaF crystallin promoter:mCherry (Crygf Mouse)
UseMulticloning site flanked by i-scei sitesTagsmCherryExpressionMutationmouse gammaF crystallin promoter (-225 - +73) pre…PromoterAvailable sinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFGL815N
Plasmid#52323PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI D-E c-myc
Plasmid#54451DepositorInsertc-MYC epitope tag (EQKLISEEDL)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI B-C c-myc
Plasmid#54450DepositorInsertc-MYC epitope tag (EQKLISEEDL)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ301
Plasmid#205415PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoterDepositorInsertHs1Cas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
plIVMD217
Plasmid#160939PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mTagBFP2-HA Tag
UseTagsHA tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS167
Plasmid#48881PurposePositive feedback plasmid from oscillatorDepositorInsertsaraC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterPara/lacAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a
Plasmid#183567PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pxpr_502-wt-ltr
Plasmid#182647Purposefor CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTRDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKDR-TetOn
Plasmid#183577PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterTet-OnAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-CBh
Plasmid#183557PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-Lenti-TetOn
Plasmid#183555PurposepKDR compatible TetOn lentiviral transfer plasmidDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS24·Pm-GFP
Plasmid#122594PurposeExpresses GFP under the regulatory control of Pm. Expression needs additional xyls geneDepositorInsertPm promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
LT0
Plasmid#210551PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporterDepositorInsertpU6::AAVS1gRNA::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT1
Plasmid#210552PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporterDepositorInsertpU6::AAVS1gRNA::LT1::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT2
Plasmid#210553PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporterDepositorInsertpU6::AAVS1gRNA::LT2::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6-spTorA-mCherry
Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
UseTags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -