We narrowed to 2,346 results for: PUC19

1. pADH143

   Plasmid

   #90989

   Purpose

   NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Zeo-ultraID

   Plasmid

   #207704

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pADH140

   Plasmid

   #90988

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   C. maltosa LEU2 1 of 2
   pENO1
   Cas9
   NAT 1 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pADH119

   Plasmid

   #90985

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLinker-AID-Ty-HXGPRT-LoxP

   Plasmid

   #86667

   Purpose

   for generation of AID strains using CRISPR tagging technology

   Depositor

   David Sibley

   Article

   Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

   Insert

   Linker-AID-Ty-HGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-N-Puro-moxGFP

   Plasmid

   #207688

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-C-miniTurbo-Zeo

   Plasmid

   #207736

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. qTAG-N-Zeo-miniTurbo

   Plasmid

   #207703

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pSZT025

   Plasmid

   #140033

   Purpose

   KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Truncated thioesterase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cLac143

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR1075

    Plasmid

    #111093

    Purpose

    Expresses eGFP donor in pUC19

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC1.530

    Plasmid

    #205441

    Purpose

    Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    RepB gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMIN-0

    Plasmid

    #197782

    Purpose

    Level 2 destination vector: minimal (non-T-DNA) backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKE014

    Plasmid

    #207908

    Purpose

    GG entry for zCas9i expression

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    SpCas9_introns_2xNLS-Pea3A(T) term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKJ237

    Plasmid

    #212320

    Purpose

    Express KnFnuc

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    KnFnuc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKJ226

    Plasmid

    #212316

    Purpose

    Express DpFnuc with pureexpress

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    DpFnuc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pADH139

    Plasmid

    #90987

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C, mal LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAM750

    Plasmid

    #207889

    Purpose

    Level 0 for A3 module (5' TCCC-3' AACA)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    dummy 20nt sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM749

    Plasmid

    #207888

    Purpose

    Level 0 for AD2 module (5' TGAC-3' TCCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    dummy 20nt sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM758

    Plasmid

    #207897

    Purpose

    Level 0 for C4 module (5' GCAG-3' TCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    dummy 20nt sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge