We narrowed to 165,694 results for: addgene

1. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLG1-puro-sgATL1-2

   Plasmid

   #109006

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL1-sgRNA #2 (ATL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19

   Plasmid

   #50005

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   lac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVLINK-EF1alpha-5' ROBO2

   Plasmid

   #236686

   Purpose

   5' AAVLINK plasmid for ROBO2 expression

   Depositor

   Zhonghua Lu

   Insert

   ROBO2 (ROBO2 Human)
   

   Use

   AAV

   Tags

   HA

   Promoter

   EF1alpha

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-MetGFP

   Plasmid

   #37560

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRMT10C (RG9MTD1A-c113)

   Plasmid

   #110248

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RG9MTD1 (TRMT10C Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-HA-CHAC1

   Plasmid

   #242710

   Purpose

   human CHAC1 gene expression

   Depositor

   Xingguo Zhu

   Insert

   CHAC1 (CHAC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Multiple wobble mutations

   Promoter

   CMV

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

   Plasmid

   #118156

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. KDM4D

    Plasmid

    #73247

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KDM4D (KDM4D Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-Tet-ON-shCtrl (neo)

    Plasmid

    #192076

    Purpose

    Lentivirus for Dox-inducible expression of a non-targeting control shRNA

    Depositor

    Roland Friedel

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Promoter

    H1 / Tet-responsive element

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid

    #99280

    Purpose

    Can be used to generate AAV virus that will express mScarlet in the presence of Cre

    Depositor

    Rylan Larsen

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7TFP

    Plasmid

    #24308

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-PuroR
    

    Use

    Lentiviral

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOK12

    Plasmid

    #49789

    Purpose

    E. coli cloning vector (KanR, low copy, blue/white selection)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    lacZ

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABEmax-SpRY

    Plasmid

    #195279

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

    Depositor

    Petros Patsali, Carsten Lederer

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

    Plasmid

    #159915

    Purpose

    Mutagenesis of Rosa26

    Depositor

    Larry Zweifel

    Insert

    Rosa26 (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK CXADR-V5 hygro

    Plasmid

    #220362

    Purpose

    Lentiviral expression vector for an inducible V5-tagged CXADR construct

    Depositor

    Kevin Janes

    Insert

    CXADR (CXADR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV CMV IGF1-FLAG

    Plasmid

    #175170

    Purpose

    Lentiviral expression of human Igf1-FLAG

    Depositor

    Larry Gerace

    Insert

    Igf1 (IGF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9_human_ADAR1_ccC

    Plasmid

    #158115

    Purpose

    deletion hADAR1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (ADAR Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9_human_ADAR1_ccB

    Plasmid

    #158116

    Purpose

    deletion hADAR1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (ADAR Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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