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Plasmid#129000PurposepREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Brr2
Plasmid#99995PurposeExpress yeast Brr2 with Y2H binding domain fusionDepositorInsertBrr2 (BRR2 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Rcl1
Plasmid#99992PurposeExpress yeast Rcl1 with Y2H activation domain fusionDepositorInsertRcl1 (RCL1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationContains amino acids 1-217 of Rcl1PromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Kre33
Plasmid#99990PurposeExpress yeast Kre33 with Y2H activation domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Kre33
Plasmid#99989PurposeExpress yeast Kre33 with Y2H binding domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ypd1p-pET22b
Plasmid#12655DepositorInsertYPD1 protein (YPD1 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorInsertHRASLS3 (PLAAT3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES2-gRNA-hyg-MCS
Plasmid#107734PurposeE. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.DepositorInsertX-3 site gRNA expression cassettes
UseCRISPRTagsExpressionYeastMutationPromoterSNR52pAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv
Plasmid#217437PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmBanana-Hyg-CTCF-CtermNES-N1
Plasmid#131851PurposeExpresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cellsDepositorInsertCTCF (CTCF Human)
UseTagsmBananaExpressionMammalianMutationthe C-terminus of CTCF is linked to HIV-Rev prote…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pp-mCAT1
Plasmid#83948PurposeThis plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectorsDepositorInsertmCAT1 (Slc7a1 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmBanana-Hyg-CTCF-NtermNES-N1
Plasmid#131850PurposeExpresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cellsDepositorInsertCTCF (CTCF Human)
UseTagsmBananaExpressionMammalianMutationdeleted amino acids 796-2181 (the N-terminus is l…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-bio-myc-NES-EGFP
Plasmid#112712PurposeFor tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cellsDepositorInsertEGFP
UseTagsHIV Rev nuclear localization signal (NES), biotin…ExpressionMammalianMutationNucleotide sequence optimized for synthetic gene …PromoterCMV with Tet repressor binding sitesAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AA054
Plasmid#215932PurposeFragmid fragment: (guide cassette) guide expression; reverse orientationDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA102
Plasmid#215935PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIsDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
RAP2A-p2A-E2Crimson
Plasmid#83940PurposeLentiviral vector expressing RAP2A-p2A-E2CrimsonDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LC-E75
Bacterial Strain#115925PurposeExpression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosomeDepositorBacterial ResistanceNoneAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEFEX3-A2aGH
Plasmid#160545PurposepYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX3-A2aGH
Plasmid#160548PurposepYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only