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Plasmid#108841PurposeInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA
Plasmid#108847PurposeInsert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA
Plasmid#108836PurposeInsert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA
Plasmid#108842PurposeInsert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBC2-PuroIII
Plasmid#153420PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
KTK_247
Plasmid#180529PurposeEntry vector containing pTac promoterDepositorInsertpTac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
STIMATE(1-240)
Plasmid#71280PurposeExpresses STIMATE or TMEM110 (1-240) in mammalian cellsDepositorInsertSTIMATE (1-240) (TMEM110 Human)
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa28_1088
Plasmid#49683DepositorInsertECF28_1088
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_436
Plasmid#49667DepositorInsertECF15_436
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Scara3-IRES-BFP
Plasmid#117265PurposeRetroviral overexpression of Scara3DepositorInsertScara3 (Scara3 Mouse)
UseRetroviralTagsExpressionMammalianMutationSee depositor comments below.PromoterLTRAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVRa11_987
Plasmid#49661DepositorInsertECF11_987
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2
Plasmid#182354PurposeNuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ro2
Plasmid#24503DepositorInsertRASSL Ro2 (OPRK1 Human)
UseTagsFLAG and NLS from prolactinExpressionMammalianMutationmutations in extracellular loops 2 (see comments …PromoterAvailable sinceJune 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA
Plasmid#108838PurposeInsert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYL,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA
Plasmid#108839PurposeInsert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA
Plasmid#108834PurposeInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBP,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Plasmid#108835PurposeInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZE21-APX(W41F) dimeric
Plasmid#58199PurposeBacterial expression of APX W41F under control of TetRDepositorInsertpea APX W41F
UseTagsExpressionBacterialMutationW41FPromoterpL(tetO-1)Available sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only