We narrowed to 1,598 results for: Tef

1. pJQR3

   Plasmid

   #218289

   Purpose

   Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITEdv1

   Plasmid

   #218283

   Purpose

   Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pITEu2

   Plasmid

   #218282

   Purpose

   Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJE13HD7

   Plasmid

   #218281

   Purpose

   Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYSD101

   Plasmid

   #212925

   Purpose

   Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SUC2 (SUC2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYSD140

   Plasmid

   #212927

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYSD113

   Plasmid

   #212926

   Purpose

   Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SUC2 (SUC2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYSD100

   Plasmid

   #212924

   Purpose

   Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SUC2 (SUC2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA.3-V5-SPOP (R221C)

   Plasmid

   #197022

   Purpose

   Mammalian expression vecor for V5-tagged SPOP

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R221C

   Promoter

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA.3-V5-SPOP (R354H)

    Plasmid

    #197023

    Purpose

    Mammalian expression vecor for V5 tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R354H

    Promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His SUMO TEV R221C SPOP 28-359

    Plasmid

    #194116

    Purpose

    R221C His SUMO SPOP expression plasmid

    Depositor

    Tanja Mittag

    Article

    Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

    Insert

    SPOP R221C (SPOP Human)
    

    Use

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    R221C

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAFMW-Nbr[R280A,K284A]

    Plasmid

    #188598

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R280A [CGA=>GCA], K284A [AAG=> GCG]

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAFMW-Nbr[K216E]

    Plasmid

    #188600

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K216E [AAA=> GAA]

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAFMW-Nbr[C399-625 CM]

    Plasmid

    #188588

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain, catalytic mutant
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    D435A [GAT=>GCC]; E435A [GAA=>GCC]

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAFMW-Nbr[C399-625]-Gwpep(Mm)

    Plasmid

    #188589

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc and GWpep mm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAFMW-Nbr[K276A]

    Plasmid

    #188597

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K276A [AAG=>GCG]

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAFMW-Nbr[C399-625]-Gwpep(Dm)

    Plasmid

    #188590

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc and Gwpep dm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAFMW-Gwpep(Dm)-Nbr[C399-625]

    Plasmid

    #188592

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc-Gwpep dm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAFMW-Gwpep(Mm)-Nbr[C399-625]

    Plasmid

    #188591

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc-Gwpep mm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAFMW-Nbr[R272A,K276A]

    Plasmid

    #188596

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge