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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-dabAB2

Plasmid

#133012

Purpose

pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC104650.1-1/1

Plasmid

#91541

Purpose

Protein expression and purification of human SH3 domain construct AC104650.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC104650.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC093799.1-1/1

Plasmid

#91495

Purpose

Protein expression and purification of human SH3 domain construct AC093799.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC093799.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-2/2

Plasmid

#91382

Purpose

Protein expression and purification of human SH3 domain construct OTOR-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-2/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-1/2

Plasmid

#91328

Purpose

Protein expression and purification of human SH3 domain construct OTOR-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-1/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP3B-1/1

Plasmid

#91299

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3B-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LOC440461-1/1

Plasmid

#91309

Purpose

Protein expression and purification of human SH3 domain construct LOC440461-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LOC440461-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_C1orf168-1/1

Plasmid

#91295

Purpose

Protein expression and purification of human SH3 domain construct C1orf168-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

C1orf168-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS09

Plasmid

#52649

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS09

Expression

Mammalian

Mutation

L414T mutated from GCaMP3

Promoter

T7

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS05

Plasmid

#52646

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS05

Expression

Mammalian

Mutation

M373Q mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS08

Plasmid

#52648

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS08

Expression

Mammalian

Mutation

T412Q/L414T mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP-cathepsinD-YMH

Plasmid

#182644

Purpose

Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

cathepsinD

Use

Retroviral

Tags

His6-tag, TS site from proCCK, and myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMRS-AraC-dCas9

Plasmid

#220195

Purpose

mini-Tn7 dCas9 expression vector - AraC/pBAD promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

AraC/pBAD

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBRC901

Plasmid

#167948

Purpose

express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Tags

hexa-histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX18-1/1

Plasmid

#91369

Purpose

Protein expression and purification of human SH3 domain construct SNX18-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX18-1/1 (SNX18 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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IBMc396

Plasmid

#161954

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAPGEF4_DEP

Plasmid

#109895

Purpose

Protein expression and purification of RAPGEF4_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAPGEF4_DEP (RAPGEF4 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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