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hMLKL-Venus-Mito

Plasmid

#106080

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Mito (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

mitochondrial-targetting domain of Bcl-xl

Expression

Mammalian

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-140)-2xFv-flag

Plasmid

#106074

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-140)-2xFv-flag (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

flag

Expression

Mammalian

Mutation

amino acids 1-140

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus-Mito

Plasmid

#106080

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Mito (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

mitochondrial-targetting domain of Bcl-xl

Expression

Mammalian

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-140)-2xFv-flag

Plasmid

#106074

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-140)-2xFv-flag (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

flag

Expression

Mammalian

Mutation

amino acids 1-140

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R269

Plasmid

#138181

Purpose

Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R268

Plasmid

#138180

Purpose

Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(LNR).gp41-8.(SAV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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p005-RFP-strong

Plasmid

#108317

Purpose

Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

Depositor

Jaroslaw Bryk

Publication

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of strong synthetic constitutive promoter pFAB4005

Expression

Bacterial

Promoter

pFAB4005

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R269

Plasmid

#138181

Purpose

Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R268

Plasmid

#138180

Purpose

Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(LNR).gp41-8.(SAV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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p005-RFP-strong

Plasmid

#108317

Purpose

Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

Depositor

Jaroslaw Bryk

Publication

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of strong synthetic constitutive promoter pFAB4005

Expression

Bacterial

Promoter

pFAB4005

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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C035

Plasmid

#174341

Purpose

Template for making Rluc mRNA reporter with pA120.

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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C035

Plasmid

#174341

Purpose

Template for making Rluc mRNA reporter with pA120.

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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4QQ6

Plasmid

#61946

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SMN1:JMC047-F11:C221089 (SMN1 Human)

Tags

6xHis and MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
prDNA spacer-LacOx120-20xMS2-Hyg

Plasmid

#210220

Purpose

Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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4QQ6

Plasmid

#61946

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SMN1:JMC047-F11:C221089 (SMN1 Human)

Tags

6xHis and MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pScaf-10080.1

Plasmid

#111410

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 10080 bases in length

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-ACKN

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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