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Showing: 301 - 350 of 8010 results

  1. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R

    Plasmid
    #221119
    Purpose
    FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-K424R
    Use
    Tags
    Expression
    Yeast
    Mutation
    STE3 mutantK424R
    Promoter
    STE3
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221119-plasmid-map-sequence-id-442458

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  30. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

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  31. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid
    #221449
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221449-plasmid-map-sequence-id-437431

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  32. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid
    #221450
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221450-plasmid-map-sequence-id-440139

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  33. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid
    #221451
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221451-plasmid-map-sequence-id-437432

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  34. 1203A-CMV-5’DR-EGFP-bGHpolyA

    Plasmid
    #221452
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221452-plasmid-map-sequence-id-437434

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  35. 1203B-CMV-3’DR-EGFP-bGHpolyA

    Plasmid
    #221453
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221453-plasmid-map-sequence-id-437437

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  36. 1203C-CMV-5’+3'DR-EGFP-bGHpolyA

    Plasmid
    #221454
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221454-plasmid-map-sequence-id-437443

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  37. 1203D-5'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221534
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221534-plasmid-map-sequence-id-437444

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  38. 1203E-3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221535
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221535-plasmid-map-sequence-id-443095

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  39. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221536
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221536-plasmid-map-sequence-id-437446

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  40. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid
    #221537
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221537-plasmid-map-sequence-id-437448

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  41. 1203Q-5'dmDR-NT(22)-GFP-bGHpolyA

    Plasmid
    #221538
    Purpose
    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221538-plasmid-map-sequence-id-442639

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  42. 1203S-5'+3’dmDR-NT(22)-GFP-bGHpolyA

    Plasmid
    #221539
    Purpose
    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' and 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221539-plasmid-map-sequence-id-437451

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  43. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  44. 1203AB-CMV-Cas7.1-H43A-mutant

    Plasmid
    #221541
    Purpose
    Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221541-plasmid-map-sequence-id-437821

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  45. 1203AC-CMV-Cas7.1-Y55A-mutant

    Plasmid
    #221542
    Purpose
    Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221542-plasmid-map-sequence-id-437820

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  46. 1203AD-CMV-Cas7.1-N152A-mutant

    Plasmid
    #221543
    Purpose
    ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221543-plasmid-map-sequence-id-437816

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  47. AAV-hSyn-PiGM-Iq(535/81, eGFP)

    Plasmid
    #221612
    Purpose
    AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Iq (535/81,eGFP)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS2 1-53 truncation
    Promoter
    Human Synapsin
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221612-plasmid-map-sequence-id-461237

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  48. AAV-hSyn-PiGM-Ii (149, 535/81, eGFP)

    Plasmid
    #221610
    Purpose
    AAV encoding the 149 amino acid version PiGM-Ii with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Ii (149, eGFP)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS10(truncation after amino acid 149)
    Promoter
    Human Synapsin
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221610-plasmid-map-sequence-id-461233

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Showing: 301 - 350 of 8010 results