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Performing In Vivo CRISPR Screens Using the FITS Approach

Type

Blog Post

...Framework for in vivo T cell screens. J Exp Med, 221(4), e20230699. https://doi.org/10.1084/jem.20230699...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

...https://www.addgene.org/136557/ 661 680 64,000 14.5 221 Monomer 980 4.5 182 emiRFP703 https://www.addgene.org...
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

Type

Blog Post

...Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620 Lu QL, Partridge TA (1998). A New Blocking...Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813 Tuson JR, Pascoe EW, Jacob DA (1990) A novel...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...memories. Cell. 2011;147(3):678-89. Pubmed PMID: 22179551. Pubmed Central PMCID: PMC4165888. 6.Witten, Ilana...reinforcement." Neuron 72.5 (2011): 721-733. PubMed PMID: 22153370. PubMed Central PMCID: PMC3282061. 7.Diester, ...
Deep Dive: qPCR

Type

Blog Post

...Quantification. Volume 14. 2017, Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001....2409 (2017). https://doi.org/10.1038/s41598-017-02217-xTaylor, SC; Nadeau, K; Abbasi, M; Lachance, C; ...
AAV Titration by qPCR Using SYBR Green Technology

Type

Protocol

...moles/μL Molecules/μl: 2.65 x 10 -13 moles/μL x 6.022145 x 10 23 molecules/mole = 1.59 x 10 11 molecules...
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

Type

Blog Post

...biotechnology 157.1 (2012): 228-236. PubMed PMID: 22154564. 6. Stoneham, Charlotte A., Michael Hollinshead...
It’s Bioelectric! An Exciting Interdisciplinary Field of Research

Type

Blog Post

...Development 139.2 (2012): 313-323. PubMed PMID: 22159581. PubMed Central PMCID: PMC3243095. Additional ...
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Type

Blog Post

...Struct. Mol. Biol. 19 (2012), 117–121. PubMed PMID: 22157958. Addgene Resources Cre-lox Resource Guide Fluorescent...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

...System." Neuron 99.3 (2018): 430-433. PubMed PMID: 30092212. PubMed Central PMCID: PMC6576255. Kyoda, Koji...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, ...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

.... 2011. PMID: 21439297. PubMed Central PMCID: PMC3622210. Schek N, Cooke C, Alwine JC. Definition of the...
The Materials Science of Optogenetics Experiments

Type

Blog Post

...-23. doi: 10.1038/nprot.2011.413. PubMed PMID: 22157972. 3. Britt JP, et al. Use of channelrhodopsin for...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

... repair pathway balance. Molecular Cell, 81(10), 2216-2230.e10. https://doi.org/10.1016/J.MOLCEL.2021.03.032...
Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

....24259. Epub 2014 Nov 21. PMID: 25418950; PMCID: PMC4312221....
An Introduction to Adenovirus

Type

Blog Post

...(1), 1679–1687. https://doi.org/10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...PLoS One, 2011. 6(11): p. e27956. PubMed PMID: 22132178. PubMed Central PMCID: PMC3222670. 10. Hong, H...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...doi.org/10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...Molecular Therapy20.2 (2012): 329-338. PubMed PMID: 22108859. PubMed Central PMCID: PMC3277219. 6. Ohmori, ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...of Sciences 109.2 (2012): 466-471. PubMed PMID: 22190486. PubMed Central PMCID: PMC3258636. Additional...

Showing: 1 - 20 of 57 results

Results per page:

Performing In Vivo CRISPR Screens Using the FITS Approach

Quick Guide to Near-Infrared Fluorescent Proteins

Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

Important Considerations in Optogenetics Behavioral Experiments

Deep Dive: qPCR

AAV Titration by qPCR Using SYBR Green Technology

Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

It’s Bioelectric! An Exciting Interdisciplinary Field of Research

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Open Resources and Plasmid Tools For Studying C. elegans

Antibodies 101: Choosing the Right Isotype

Plasmids 101: Terminators and PolyA signals

The Materials Science of Optogenetics Experiments

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Antibodies 101: Producing Recombinant Antibodies

An Introduction to Adenovirus

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Plasmids 101: Plasmid Incompatibility

Adeno-associated Viruses (AAVs) for Genome Editing

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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