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We narrowed to 1,068 results for: golden gate assembly

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Showing: 301 - 350 of 1068 results

  5. mUAVbla:tetR 5′

    Plasmid
    #149379
    Purpose
    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Insert
    tetA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149379-plasmid-map-sequence-id-308791

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  6. (P1)PA1lacO1

    Plasmid
    #251365
    Purpose
    IPTG-inducible Plac promoter variant with lacIq repressor and RiboJ
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    Promoter A1lacO-1
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251365-plasmid-map-sequence-id-503679

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  7. (P6)RBSS3

    Plasmid
    #251388
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251388-plasmid-map-sequence-id-503708

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  8. (P4)RBSS1

    Plasmid
    #251376
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251376-plasmid-map-sequence-id-503738

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  9. (P6)RBSS1

    Plasmid
    #251386
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251386-plasmid-map-sequence-id-503734

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  10. (P2)RBSS1

    Plasmid
    #251366
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251366-plasmid-map-sequence-id-503714

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  11. (P2)RBSS3

    Plasmid
    #251368
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251368-plasmid-map-sequence-id-503703

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  12. (P4)RBSS3

    Plasmid
    #251378
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251378-plasmid-map-sequence-id-503698

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  13. (P4)RBSS4

    Plasmid
    #251379
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251379-plasmid-map-sequence-id-503681

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  14. (P6)RBSS4

    Plasmid
    #251389
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251389-plasmid-map-sequence-id-503678

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  15. (P2)RBSS4

    Plasmid
    #251369
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251369-plasmid-map-sequence-id-503676

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  16. pAV10.KH

    Plasmid
    #63208
    Purpose
    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    63208-plasmid-map-sequence-id-104909

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  17. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  18. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  19. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  20. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  21. pAV113.loxP

    Plasmid
    #63186
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63186-plasmid-map-sequence-id-131462

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  22. pAV115.loxP

    Plasmid
    #63187
    Purpose
    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63187-plasmid-map-sequence-id-104891

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  23. pAV116.loxP

    Plasmid
    #63188
    Purpose
    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63188-plasmid-map-sequence-id-121508

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  24. pAV10.K3.loxP

    Plasmid
    #63202
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63202-plasmid-map-sequence-id-104899

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  25. pAV10.HO6

    Plasmid
    #63211
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63211-plasmid-map-sequence-id-115802

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  26. (P5)pdc

    Plasmid
    #251385
    Purpose
    Gene encoding pyruvate decarboxylase (Zymomonas mobilis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    pdc
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251385-plasmid-map-sequence-id-503707

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  27. (P4)TT

    Plasmid
    #253857
    Purpose
    rrnB T1 terminator derived from pTrc99A
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    rrnB T1 terminator derived from pTrc99A
    Expression
    Bacterial
    Available Since
    June 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253857-plasmid-map-sequence-id-503687

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  28. (P4)RBSS2

    Plasmid
    #251377
    Purpose
    RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S2
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251377-plasmid-map-sequence-id-503740

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  29. (P6)RBSS2

    Plasmid
    #251387
    Purpose
    RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S2
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251387-plasmid-map-sequence-id-503742

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  30. (P3)Adh

    Plasmid
    #251375
    Purpose
    Gene encoding aldehyde reductase slr1192 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    adh
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251375-plasmid-map-sequence-id-503747

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  31. (P2)RBSS2

    Plasmid
    #251367
    Purpose
    RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S2
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251367-plasmid-map-sequence-id-503730

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  32. (P7)Pk

    Plasmid
    #251395
    Purpose
    Gene encoding pyruvate kinase (Enterococcus faecalis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    Pyruvate kinase
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251395-plasmid-map-sequence-id-503705

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  33. (P4)RBSB

    Plasmid
    #251383
    Purpose
    RBS designed in silico [75] for high expression in E. coli
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS B
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251383-plasmid-map-sequence-id-503702

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  34. (P2)RBSB

    Plasmid
    #251373
    Purpose
    RBS designed in silico [75] for high expression in E. coli
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS B
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251373-plasmid-map-sequence-id-503677

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  35. (P6)RBSB

    Plasmid
    #251393
    Purpose
    RBS designed in silico [75] for high expression in E. coli
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS B
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251393-plasmid-map-sequence-id-503674

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Showing: 301 - 350 of 1068 results