1. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pBSV2_P0026-mCherryBb

   Plasmid

   #118239

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   bb0026 promoter driven mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b-Casp8 delta DED

   Plasmid

   #11827

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 8 minus Death Effecter Domain (CASP8 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Minus Death Defecter Domain (aa 1-216)

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pBSV2G_2

   Plasmid

   #118225

   Purpose

   Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-DYRK1A-2A-GFP

   Plasmid

   #118270

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV with beta global intron

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBSV2H

   Plasmid

   #118229

   Purpose

   Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBSV2G_PflaB-msfYFPBb

   Plasmid

   #118233

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfYFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBSV2G_PflaB-mCeruleanBb

   Plasmid

   #118235

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven mCerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-OTUB1 T134R-pcDNA3.1

   Plasmid

   #118211

   Purpose

   Expresses FLAG-OTUB1 T134R mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 134 to arginine

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

   Plasmid

   #118274

   Purpose

   Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Inserts

   bPAC(WT)
   SthK
   

   Use

   AAV

   Tags

   His tag, P2A, mCherry, and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CamK II

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBSV2_2

    Plasmid

    #118226

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJS102

    Plasmid

    #118281

    Purpose

    Expresses GFPmut2 with low cell-to-cell variation and lactose induction

    Depositor

    Zach Hensel

    Article

    Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UAS:Zebrabow-V

    Plasmid

    #118218

    Purpose

    For broad or variegated gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Alex Schier, Albert Pan

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-DYRK2-2A-mCherry

    Plasmid

    #118285

    Purpose

    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-DCX(S306D)-2A-mCherry

    Plasmid

    #118292

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Serine 306 changed to Aspartic Acid

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-DCX(S306A)-2A-mCherry

    Plasmid

    #118291

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    changed Serine 306 to Alanine

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3xHA-BioID_pAS28

    Plasmid

    #118223

    Purpose

    expresses 3xHA-tagged BioID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    BioID (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    R118G

    Promoter

    ges-1p

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-ERK2 T183AY185F

    Plasmid

    #118299

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 T183AY185F (Mapk1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T183AY185F

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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