Skip to main content
Addgene
Showing: 121 - 140 of 825 results

  4. pINDUCER20-hSRF-3xHA (#757)

    Plasmid
    #78344
    Purpose
    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pMXs-hs-3xHA-7CtoA-LIN-41

    Plasmid
    #52726
    Purpose
    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags
    Depositor
    Shinya Yamanaka
    Article
    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.
    Insert
    LIN-41 (TRIM71 Human)
    Use
    Retroviral
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    changed cysteines 12, 15, 61, 66, 69, 91, and 94 …
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52726-plasmid-map-sequence-id-82095

    Enlarge

  6. pMXs-hs-3xHA-C12AC15A-LIN-41

    Plasmid
    #52725
    Purpose
    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags
    Depositor
    Shinya Yamanaka
    Article
    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.
    Insert
    LIN-41 (TRIM71 Human)
    Use
    Retroviral
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    changed cysteines 12 and 15 to alanines
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52725-plasmid-map-sequence-id-82094

    Enlarge

  10. pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227906
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    227906-plasmid-map-sequence-id-458972

    Enlarge

  11. DEXsynth:3xhA-ccdb + RUBY

    Plasmid
    #233318
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:3xhA-ccdb + RUBY
    Use
    Gateway destination
    Tags
    3xHA
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233318-plasmid-map-sequence-id-464361

    Enlarge

  12. Ubi:3xHA-ccdb + RUBY

    Plasmid
    #233307
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:3xHA-ccdb + RUBY
    Use
    Gateway destination
    Tags
    3xHA
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233307-plasmid-map-sequence-id-464373

    Enlarge

  13. DEXsynth:3xhA-ccdb + mcherry

    Plasmid
    #233313
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:3xhA-ccdb + mcherry
    Use
    Gateway destination
    Tags
    3xHA
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233313-plasmid-map-sequence-id-464360

    Enlarge

  14. Ubi:3xHA-ccdb + mcherry

    Plasmid
    #233301
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker
    Depositor
    Thomas Baum
    Insert
    Ubi:3xHA-ccdb + mcherry
    Use
    Gateway destination
    Tags
    3xHA
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233301-plasmid-map-sequence-id-462180

    Enlarge

  15. pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227923
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227923-plasmid-map-sequence-id-456037

    Enlarge

  16. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227925
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227925-plasmid-map-sequence-id-456028

    Enlarge

  17. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227926
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227926-plasmid-map-sequence-id-452495

    Enlarge

  18. pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227908
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227908-plasmid-map-sequence-id-452488

    Enlarge

  19. pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227909
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227909-plasmid-map-sequence-id-452501

    Enlarge

  20. pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227910
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227910-plasmid-map-sequence-id-456010

    Enlarge

Showing: 121 - 140 of 825 results