Addgene

We narrowed to 6,281 results for: KRAS

Showing: 121 - 140 of 6281 results

Results per page:

Sort results by:

pB-CAGGS-dCas9-KRAB

Plasmid

#110822

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Use

Tags

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-tTRKRAB-red

Plasmid

#12250

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

TetR-KRAB fusion and dsRED

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

dsRed added to backbone.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-KRAS-G12V

Plasmid

#116424

Purpose

Lentiviral expression of KRAS G12V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Kras (G12V)-pcw107

Plasmid

#64602

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AKAR4-Kras

Plasmid

#61621

Purpose

Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4-Kras

Use

Tags

C-terminal targeting sequence from Kras, Cerulean…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVPRT-tTR-KRAB

Plasmid

#11648

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hPrion, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-Kras S17N

Plasmid

#58899

Purpose

Expresses human Kras with a S17N mutation

Depositor

Channing Der

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

S17N mutation

Promoter

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXEBR-pTF-kRasV12

Plasmid

#114318

Purpose

TET-inducible expression of the V12-mutant form of the ki-Ras protein

Depositor

Patrick Salmon

Insert

Ras (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V oncogenic mutation. Constitutively active K-…

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-YFP-KRAS4B

Plasmid

#112718

Purpose

Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

Depositor

Kenneth Westover

Article

Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

Insert

YFP-KRAS4B (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVUHshp53-tTR-KRAB

Plasmid

#11654

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVPT-rtTR-KRAB

Plasmid

#11777

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-HA-2xMCP-KRAB

Plasmid

#126589

Purpose

Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-KRAB

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pHR-FKBP:mCherry-KRas4Bc30

Plasmid

#72900

Purpose

Membrane-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

GTPase K-Ras (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158..188 (fragment 1..157 deleted)

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
8798 GFP-KrasG12C_2B_hygro

Plasmid

#64376

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

changed glycine 12 to cysteine

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge