We narrowed to 12,255 results for: cel.2

1. Myc-PSFΔRRM1+2

   Plasmid

   #35267

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   PSF (SFPQ Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of residues 298-429

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pBS_KS_attB2_SA(2)_T2AGAL4

   Plasmid

   #125213

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL4
   

   Use

   Other

   Tags

   T2A

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDTnLAPP2A frame 2

   Plasmid

   #28228

   Depositor

   Harald von Melchner

   Article

   Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

   Insert

   Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   EGFP and nLAP-tag

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-homo_PTGS1-2

   Plasmid

   #184732

   Purpose

   COX1-KO in HeLa

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the human COX1 gene.
   

   Use

   CRISPR

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-homo_PLA2G4A-2

   Plasmid

   #184736

   Purpose

   cPLA2-KO in HeLa

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the human cPLA2 gene.
   

   Use

   CRISPR

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBzCas13b-gRNA-2

   Plasmid

   #164859

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-2
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLsCas13a-gRNA-2

   Plasmid

   #164866

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-2
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS_KS_attB2_SA(2)_T2AGeneSwitch

   Plasmid

   #125216

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GeneSwitch
   

   Use

   Other

   Tags

   T2A

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS_KS_attB2_SA(2)_T2AGAL80

   Plasmid

   #125219

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL80
   

   Use

   Other

   Tags

   T2A

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-sgAAVS1-2

    Plasmid

    #129727

    Purpose

    Expressing SpCas9 and sgAAVS1-2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-2
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-SREBP-2

    Plasmid

    #204200

    Purpose

    Mammalian expression of SREBP-2

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-2 (SREBF2 Human)
    

    Tags

    T7x3

    Expression

    Mammalian

    Mutation

    silent mutations

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYU-2-Env

    Plasmid

    #133997

    Purpose

    Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

    Depositor

    Himanshu Garg

    Article

    Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

    Insert

    Envelope Glycoprotein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pegRNA_LMNA_CR-2-5

    Plasmid

    #199275

    Purpose

    pegRNA used to correct LMNA (c.1824C > T) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LMNA 1824TtoC pegRNA
    

    Expression

    Mammalian

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. psiTE(2)-Gal4

    Plasmid

    #220451

    Purpose

    Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Expression

    Insect

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiTE(2)-Gal4DBD

    Plasmid

    #220454

    Purpose

    Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4DBD-CfaC
    

    Expression

    Insect

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiTE(2)-LexAGAD

    Plasmid

    #220460

    Purpose

    Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-LexAGAD-CfaC
    

    Expression

    Insect

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. psiTE(2)-p65AD

    Plasmid

    #220457

    Purpose

    Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-p65AD-CfaC
    

    Expression

    Insect

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. psiT-GEM(2)

    Plasmid

    #220448

    Purpose

    Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Expression

    Insect

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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